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WB (Western Blot)
(Detection of human ALDH1A1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, MCF-7, Hep-G2, Jurkat, SK-MEL-28, GaMG and (10 ug) A-549 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-ALDH1A1 recombinant monoclonal antibody (AAA213569 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Cytoskeletal Actin recombinant monoclonal antibody .)
ALDH1A1 recombinant antibody
Rabbit anti-ALDH1A1 Recombinant Monoclonal Antibody [BLR089G]
Gene Names
ALDH1A1; ALDC; ALDH1; HEL-9; HEL12; PUMB1; ALDH11; RALDH1; ALDH-E1; HEL-S-53e
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
ALDH1A1, Recombinant Antibody; Rabbit anti-ALDH1A1 Recombinant Monoclonal Antibody [BLR089G]; aldehyde dehydrogenase, liver cytosolic; epididymis luminal protein 12; retinal dehydrogenase 1; RALDH1; RALDH 1; PUMB1; HEL-S-53e; HEL-9; HEL12; epididymis secretory sperm binding protein Li 53e; epididymis luminal protein 9; Aldehyde dehydrogenase family 1 member A1; acetaldehyde dehydrogenase 1; ALDH1; aldehyde dehydrogenase 1, soluble; ALHDII; Aldehyde dehydrogenase, cytosolic; retinaldehyde dehydrogenase 1; ALDH class 1; ALDH11; ALDH-E1; ALDC; Retinal dehydrogenase 1; aldehyde dehydrogenase 1 family member A1; ALDH1A1 recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR089G
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
500ug/ml (varies by lot)
Applicable Applications for ALDH1A1 recombinant antibody
ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 325 and 375
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human ALDH1A1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, MCF-7, Hep-G2, Jurkat, SK-MEL-28, GaMG and (10 ug) A-549 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-ALDH1A1 recombinant monoclonal antibody (AAA213569 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Cytoskeletal Actin recombinant monoclonal antibody .)
WB (Western Blot)
(Detection of human ALDH1A1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, MCF-7, Hep-G2, Jurkat, SK-MEL-28, GaMG and (10 ug) A-549 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-ALDH1A1 recombinant monoclonal antibody (AAA213569 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Cytoskeletal Actin recombinant monoclonal antibody .)
IP (Immunoprecipitation)
(Detection of human ALDH1A1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from A-549 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-ALDH1A1 recombinant monoclonal antibody (AAA213569 lot 1) used for IP at 12 ul per reaction. ALDH1A1 was also immunoprecipitated by rabbit anti-ALDH1A1 recombinant monoclonal antibody [BL-1250-2E7]. For blotting immunoprecipitated ALDH1A1, AAA213569 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human ALDH1A1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from A-549 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-ALDH1A1 recombinant monoclonal antibody (AAA213569 lot 1) used for IP at 12 ul per reaction. ALDH1A1 was also immunoprecipitated by rabbit anti-ALDH1A1 recombinant monoclonal antibody [BL-1250-2E7]. For blotting immunoprecipitated ALDH1A1, AAA213569 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemisry)
(Detection of human ALDH1A1 in FFPE lung carcinoma by IHC. Antibody: Rabbit anti-ALDH1A1 recombinant monoclonal antibody (AAA213569 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemisry)
(Detection of human ALDH1A1 in FFPE lung carcinoma by IHC. Antibody: Rabbit anti-ALDH1A1 recombinant monoclonal antibody (AAA213569 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohiostchemistry)
(Detection of human ALDH1A1 in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-ALDH1A1 recombinant monoclonal antibody (AAA213569 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohiostchemistry)
(Detection of human ALDH1A1 in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-ALDH1A1 recombinant monoclonal antibody (AAA213569 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human ALDH1A1 in FFPE NCI-H2286 cells by ICC. Antibody: Rabbit anti-ALDH1A1 recombinant monoclonal antibody (AAA213569 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human ALDH1A1 in FFPE NCI-H2286 cells by ICC. Antibody: Rabbit anti-ALDH1A1 recombinant monoclonal antibody (AAA213569 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
Related Product Information for ALDH1A1 recombinant antibody
ALDH1A1 is a liver cytosolic form of acetaldehyde dehydrogenase and is involved in the major pathway of alcohol metabolism. ALDH1A1 is also known as retinal dehydrogenase 1 and is involved in retinol metabolism. [taken from NCBI Entrez Gene (Gene ID: 216)].
Product Categories/Family for ALDH1A1 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
54,862 Da
NCBI Official Full Name
retinal dehydrogenase 1
NCBI Official Synonym Full Names
aldehyde dehydrogenase 1 family, member A1
NCBI Official Symbol
ALDH1A1
NCBI Official Synonym Symbols
ALDC; ALDH1; HEL-9; HEL12; PUMB1; ALDH11; RALDH1; ALDH-E1; HEL-S-53e
NCBI Protein Information
retinal dehydrogenase 1; ALDH class 1; ALHDII; RALDH 1; acetaldehyde dehydrogenase 1; aldehyde dehydrogenase 1, soluble; aldehyde dehydrogenase, liver cytosolic; epididymis luminal protein 12; epididymis luminal protein 9; epididymis secretory sperm bindi
UniProt Protein Name
Retinal dehydrogenase 1
UniProt Gene Name
ALDH1A1
UniProt Synonym Gene Names
ALDC; ALDH1; PUMB1; RALDH 1; RalDH1
UniProt Entry Name
AL1A1_HUMAN
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Product Notes
The ALDH1A1 aldh1a1 (Catalog #AAA213569) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-ALDH1A1 Recombinant Monoclonal Antibody [BLR089G] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ALDH1A1 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the ALDH1A1 aldh1a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ALDH1A1, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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