IHC (Immunohistochemistry)
(Alpha smooth muscle actin positivity in the vessel walls of submucosa of small intestine tissue. Formalin fixed, paraffin embedded human tissue (4 ?m section) stained with AAA79010 monospecific antibody according to related datasheet.)
Rabbit anti-Human Alpha Smooth Muscle Actin Monoclonal Antibody | anti-ACTA2 antibody
Anti - Alpha Smooth Muscle Actin
Gene Names
ACTA2; AAT6; ACTSA; MYMY5
Reactivity
Human
Applications
Immunohistochemistry
Synonyms
Alpha Smooth Muscle Actin, Antibody; Anti - Alpha Smooth Muscle Actin; anti-ACTA2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
M16-L
Specificity
Human, mouse
Sequence Length
377
Applicable Applications for anti-ACTA2 antibody
IHC (Immunohistochemistry)
Immunogen
Peptide derived from the N-terminal sequence of human alpha smooth muscle actin. Antibody recognizes the epitope between Glu3 - Gly16.
Cellular localization
cytoplasm, cytoskeleton
Positive control
tumors arising from smooth muscles and myoepithelial cells
Storage Buffer
20 mM Tris-HCI, pH 8.0
Stabilizer
20 mg/ml BSA
Preservative
0.05% NaN3
IHC-P Protocol - Instruction Manual
1. Deparaffinize the section in 3 changes of xylene, 10 minutes each.
2. Wash the section in 96%, 80% and 70% ethyl alcohol for 10 minutes each.
3. Rinse in distilled water, 2 x 5 minutes.
4. Block the endogenous peroxidase by incubating the tissue in 3% hydrogen peroxide (H2O2) for 10 minutes.
5. Wash in distilled water, 2 x 5 minutes.
6. For antigen retrieval: Immerse the slide in Tris-EDTA buffer, pH 9.0* and incubate at 95-97ºC in water bath for 20 minutes.
7. Remove the staining to room temperature and let the slide to cool (in Tris-EDTA buffer, pH 9.0) for 15 minutes.
8. Rinse in distilled water, 2 x 5 minutes.
9. Wash in PBS (phosphate buffer saline, pH 7.0-7.5) supplemented with 0.05% of Tween-20 (buffer A), 2 x 5 minutes
10. CONCENTRATED:
Incubate the section with primary antibody at the dilution 1:100 – 1:200 for 1 hour in the closed wet chamber.
READY TO USE (RTU):
Incubate the section with primary antibody (ready to use) for 1 hour in a closed wet chamber.
11. Wash 3 x 5 minutes with buffer A.
12. Apply the secondary antibody (the protocol depends on the supplier), and proceed to standard immunohistochemistry protocol (HRP - Peroxide - DAB). Micropolymer-HRP detection kit rabbit/mouse dual is suggested.
13. Wash 3 x 5 minutes with buffer A.
14. Apply the chromogen (DAB), 1 - 3 minutes.
15. Wash in water, 2 x 5 minutes.
16. Stain in hematoxylin for 5 minutes.
17. Wash in distilled water, 3 x 2 minutes.
18. Mount the slide for observation.
* Tris-EDTA Buffer (10mM Tris Base, 1mM EDTA solution, 0.05% Tween-20, pH 9.0):
Tris ----------- 1.21 g; EDTA ---------- 0.37 g; Distilled water ------------ 1000 ml
Mix to dissolve in 700 ml of distilled water. Adjust pH to 9.0 with 1M HCl and then add 0.5 ml of Tween-20 and mix well. Adjust the final volume to 1 liter with distilled water.
Store this solution at room temperature for 3 months or at +4ºC for longer storage.
2. Wash the section in 96%, 80% and 70% ethyl alcohol for 10 minutes each.
3. Rinse in distilled water, 2 x 5 minutes.
4. Block the endogenous peroxidase by incubating the tissue in 3% hydrogen peroxide (H2O2) for 10 minutes.
5. Wash in distilled water, 2 x 5 minutes.
6. For antigen retrieval: Immerse the slide in Tris-EDTA buffer, pH 9.0* and incubate at 95-97ºC in water bath for 20 minutes.
7. Remove the staining to room temperature and let the slide to cool (in Tris-EDTA buffer, pH 9.0) for 15 minutes.
8. Rinse in distilled water, 2 x 5 minutes.
9. Wash in PBS (phosphate buffer saline, pH 7.0-7.5) supplemented with 0.05% of Tween-20 (buffer A), 2 x 5 minutes
10. CONCENTRATED:
Incubate the section with primary antibody at the dilution 1:100 – 1:200 for 1 hour in the closed wet chamber.
READY TO USE (RTU):
Incubate the section with primary antibody (ready to use) for 1 hour in a closed wet chamber.
11. Wash 3 x 5 minutes with buffer A.
12. Apply the secondary antibody (the protocol depends on the supplier), and proceed to standard immunohistochemistry protocol (HRP - Peroxide - DAB). Micropolymer-HRP detection kit rabbit/mouse dual is suggested.
13. Wash 3 x 5 minutes with buffer A.
14. Apply the chromogen (DAB), 1 - 3 minutes.
15. Wash in water, 2 x 5 minutes.
16. Stain in hematoxylin for 5 minutes.
17. Wash in distilled water, 3 x 2 minutes.
18. Mount the slide for observation.
* Tris-EDTA Buffer (10mM Tris Base, 1mM EDTA solution, 0.05% Tween-20, pH 9.0):
Tris ----------- 1.21 g; EDTA ---------- 0.37 g; Distilled water ------------ 1000 ml
Mix to dissolve in 700 ml of distilled water. Adjust pH to 9.0 with 1M HCl and then add 0.5 ml of Tween-20 and mix well. Adjust the final volume to 1 liter with distilled water.
Store this solution at room temperature for 3 months or at +4ºC for longer storage.
Precautions
1. We strongly recommend to use the Primary Antibody Diluent (catalog number: Please inquire), eventually alternative diluent (containing protease free BSA at the concentrations > = 1mg/ml) for dilution of concentrated antibodies, otherwise the warranty might be voided.
2. Centrifuge the vial before use.
3. Intended for research use.
4. Do not use after expiration date stamped on vial label.
5. Avoid contamination of the reagent.
6. Any discrepancies in the recommended procedures stated in the working protocol may affect the final results.
7. The reagent contains sodium azide (NaN3) which is highly toxic in higher concentrations. The concentration in the reagent (0.05%) is not considered as hazardous.
8. Disposal of waste material must be conducted in accordance with local regulations.
9. Wear appropriate Personal Protective Equipment to avoid contact with eyes and skin.
2. Centrifuge the vial before use.
3. Intended for research use.
4. Do not use after expiration date stamped on vial label.
5. Avoid contamination of the reagent.
6. Any discrepancies in the recommended procedures stated in the working protocol may affect the final results.
7. The reagent contains sodium azide (NaN3) which is highly toxic in higher concentrations. The concentration in the reagent (0.05%) is not considered as hazardous.
8. Disposal of waste material must be conducted in accordance with local regulations.
9. Wear appropriate Personal Protective Equipment to avoid contact with eyes and skin.
Preparation and Storage
Store at +4 degree C.
IHC (Immunohistochemistry)
(Alpha smooth muscle actin positivity in the vessel walls of submucosa of small intestine tissue. Formalin fixed, paraffin embedded human tissue (4 ?m section) stained with AAA79010 monospecific antibody according to related datasheet.)
IHC (Immunohistochemistry)
(Alpha smooth muscle actin positivity in the vessel walls of submucosa of small intestine tissue. Formalin fixed, paraffin embedded human tissue (4 ?m section) stained with AAA79010 monospecific antibody according to related datasheet.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Actin, aortic smooth muscle
NCBI Official Synonym Full Names
actin, alpha 2, smooth muscle, aorta
NCBI Official Symbol
ACTA2
NCBI Official Synonym Symbols
AAT6; ACTSA; MYMY5
NCBI Protein Information
actin, aortic smooth muscle; alpha-cardiac actin; cell growth-inhibiting gene 46 protein
UniProt Protein Name
Actin, aortic smooth muscle
UniProt Gene Name
ACTA2
UniProt Synonym Gene Names
ACTSA; ACTVS
UniProt Entry Name
ACTA_HUMAN
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Product Notes
The ACTA2 acta2 (Catalog #AAA79010) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti - Alpha Smooth Muscle Actin reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Alpha Smooth Muscle Actin can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the ACTA2 acta2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Alpha Smooth Muscle Actin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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