Application Data
(Mousegutsamplesstainedwith2F11at1:1000dilutionwithovernightincubationat4°CinPBSwith0.1%BSA,Secondarybiotinylatedanti-mouseantibodyfor1houratRTalsoin0.1%BSAPBS.VECTASTAINABCreagentkitwasaddedaftersecondaryincubation.PeroxidaseHRPsubstratewasleftontheguttissuefor6minutes.H&Estainingofalltissuewasperformedat1:1withdH20,rinsed,thenstainedwithblueingsolutionpriortodehydration.Imagingat40xmagnification.MousetissueusedfromamouseParkinson’sdisease(PD)seedingmodelbasedonC57BLJ/6micewithbraininjectionsofHumanPDpatientalpha-synucleinfractions.Mousegutsectionswereusedatathicknessof3µm.Toppanelshows(inbiologicalorderofmousegutfromileumtodescendingcolon)controlsecondarystaining.BottompanelshowsgutsamplesfrommiceinjectedwithhumanPDpatientbrain-derivedalphasynuclein,alsoinbiologicalorder(ileumtodescendingcolon).Thereisclearaggregatestainingvisibleinthelowerpanel.)
Mouse anti-Human, Mouse Alpha Synuclein Antibody (Aggregate-Specific) Monoclonal Antibody | anti-Snca antibody
Alpha Synuclein Antibody (Aggregate-Specific)
Application Data
(Mousegutsamplesstainedwith2F11at1:1000dilutionwithovernightincubationat4°CinPBSwith0.1%BSA,Secondarybiotinylatedanti-mouseantibodyfor1houratRTalsoin0.1%BSAPBS.VECTASTAINABCreagentkitwasaddedaftersecondaryincubation.PeroxidaseHRPsubstratewasleftontheguttissuefor6minutes.H&Estainingofalltissuewasperformedat1:1withdH20,rinsed,thenstainedwithblueingsolutionpriortodehydration.Imagingat40xmagnification.MousetissueusedfromamouseParkinson’sdisease(PD)seedingmodelbasedonC57BLJ/6micewithbraininjectionsofHumanPDpatientalpha-synucleinfractions.Mousegutsectionswereusedatathicknessof3µm.Toppanelshows(inbiologicalorderofmousegutfromileumtodescendingcolon)controlsecondarystaining.BottompanelshowsgutsamplesfrommiceinjectedwithhumanPDpatientbrain-derivedalphasynuclein,alsoinbiologicalorder(ileumtodescendingcolon).Thereisclearaggregatestainingvisibleinthelowerpanel.)
Application Data
(Mousegutsamplesstainedwith2F11at1:1000dilutionwithovernightincubationat4°CinPBSwith0.1%BSA,Secondarybiotinylatedanti-mouseantibodyfor1houratRTalsoin0.1%BSAPBS.VECTASTAINABCreagentkitwasaddedaftersecondaryincubation.PeroxidaseHRPsubstratewasleftontheguttissuefor6minutes.H&Estainingofalltissuewasperformedat1:1withdH20,rinsed,thenstainedwithblueingsolutionpriortodehydration.Imagingat40xmagnification.MousetissueusedfromamouseParkinson’sdisease(PD)seedingmodelbasedonC57BLJ/6micewithbraininjectionsofHumanPDpatientalpha-synucleinfractions.Mousegutsectionswereusedatathicknessof3µm.Toppanelshows(inbiologicalorderofmousegutfromileumtodescendingcolon)controlsecondarystaining.BottompanelshowsgutsamplesfrommiceinjectedwithhumanPDpatientbrain-derivedalphasynuclein,alsoinbiologicalorder(ileumtodescendingcolon).Thereisclearaggregatestainingvisibleinthelowerpanel.)
Application Data
(8DaydifferentiatediPSCswithaddedalphasynucleinfibrils ,conjugatedtoAlexaFluor555withorwithout1hprior2F11addition.AlexaFluor555addedat5µg/wellfor48h,2F11(50ug/welladded1hbeforefibrilswhereapplicable).Cellsweresubsequentlywashed48hafteradditionandimagedafollowing24hlater.Imagingandchannels:2F11antibody:647nm(usingananti-mouse-647conjugate);Fibrils:555nm,phosph-ser129 488nm,andHoechststainforDNA.Image4atop,nofibrils,no2F11added.Bottom,fibrilsadded,no2F11antibody.Fibrilsareclearlyvisibleinthecellsasisenhancedphosph-ser129signal.Image4btop,nofibrils,2F11antibodyadded.Bottom,fibrilsand2F11antibodyadded.Theimageclearlyshows2F11antibodyadditionstopscellentryofthe555-labelledfibrils.)
Application Data
(8DaydifferentiatediPSCswithaddedalphasynucleinfibrils ,conjugatedtoAlexaFluor555withorwithout1hprior2F11addition.AlexaFluor555addedat5µg/wellfor48h,2F11(50ug/welladded1hbeforefibrilswhereapplicable).Cellsweresubsequentlywashed48hafteradditionandimagedafollowing24hlater.Imagingandchannels:2F11antibody:647nm(usingananti-mouse-647conjugate);Fibrils:555nm,phosph-ser129 488nm,andHoechststainforDNA.Image4atop,nofibrils,no2F11added.Bottom,fibrilsadded,no2F11antibody.Fibrilsareclearlyvisibleinthecellsasisenhancedphosph-ser129signal.Image4btop,nofibrils,2F11antibodyadded.Bottom,fibrilsand2F11antibodyadded.Theimageclearlyshows2F11antibodyadditionstopscellentryofthe555-labelledfibrils.)
Application Data
(SPRbindinganalysisof2F11antibodyandhumanalphasynucleintype1fibrils ,type2 fibrilsandmonomericprotein .Buffer(topleft),alphasynucleinmonomers(topright),alphasynucleinfibrilstype1(bottomleft),andalphasynucleinfibrilstype2(bottomright).Data:KD[nM]=42.5,365.3;2F11binds with8.6xgreateraffinitythan doesnotbindmonomericalphasynuclein .)
Application Data
(SPRbindinganalysisof2F11antibodyandhumanalphasynucleintype1fibrils ,type2 fibrilsandmonomericprotein .Buffer(topleft),alphasynucleinmonomers(topright),alphasynucleinfibrilstype1(bottomleft),andalphasynucleinfibrilstype2(bottomright).Data:KD[nM]=42.5,365.3;2F11binds with8.6xgreateraffinitythan doesnotbindmonomericalphasynuclein .)
WB (Western Blot)
(Westernbloton15ugHelalysate.Block:5%SkimMilkin1XTBST.PrimaryAntibody:MouseAnti-AlphaSynucleinMonoclonalAntibodyclone2F11at1:1000for2hoursatRT.SecondaryAntibody:GoatAnti-MouseHRP:IgGat1:3000for1houratRT.ColorDevelopment:ECLsolution(SuperSignalWestPico)for5mininRT.Bandclearlyidentifieslargemolecularweightoligomericband(monomericbandpredictedat14kD).)
WB (Western Blot)
(Westernbloton15ugHelalysate.Block:5%SkimMilkin1XTBST.PrimaryAntibody:MouseAnti-AlphaSynucleinMonoclonalAntibodyclone2F11at1:1000for2hoursatRT.SecondaryAntibody:GoatAnti-MouseHRP:IgGat1:3000for1houratRT.ColorDevelopment:ECLsolution(SuperSignalWestPico)for5mininRT.Bandclearlyidentifieslargemolecularweightoligomericband(monomericbandpredictedat14kD).)
WB (Western Blot)
(Westernbloton15ugmousebrainlysatefromFmr1KOmice(FXSmice)withC57Bl/6Jgeneticbackground.Block:5%SkimMilkin1XTBST.PrimaryAntibody:MouseAnti-AlphaSynucleinMonoclonalAntibodyclone2F11at1:1000for2hoursatRT.SecondaryAntibody:GoatAnti-MouseHRP:IgGat1:3000for1houratRT.ColorDevelopment:ECLsolution(SuperSignalWestPico)for5mininRT.Bandclearlyidentifieslargemolecularweightoligomericband(monomericbandpredictedat14kD).)
WB (Western Blot)
(Westernbloton15ugmousebrainlysatefromFmr1KOmice(FXSmice)withC57Bl/6Jgeneticbackground.Block:5%SkimMilkin1XTBST.PrimaryAntibody:MouseAnti-AlphaSynucleinMonoclonalAntibodyclone2F11at1:1000for2hoursatRT.SecondaryAntibody:GoatAnti-MouseHRP:IgGat1:3000for1houratRT.ColorDevelopment:ECLsolution(SuperSignalWestPico)for5mininRT.Bandclearlyidentifieslargemolecularweightoligomericband(monomericbandpredictedat14kD).)
Application Data
(Immuno-goldelectronmicrographofsonicated?-SynFibrils stainedwith2F11antibody(1:400dilution)andvisualizedusinggoatanti-mouseIgGconjugatedwith18nmgoldparticles.Thesamplewasthenplacedonatransmissionelectronmicroscopymetallicgridandstainedwithuranylacetate.)
Application Data
(Immuno-goldelectronmicrographofsonicated?-SynFibrils stainedwith2F11antibody(1:400dilution)andvisualizedusinggoatanti-mouseIgGconjugatedwith18nmgoldparticles.Thesamplewasthenplacedonatransmissionelectronmicroscopymetallicgridandstainedwithuranylacetate.)
NCBI and Uniprot Product Information
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Product Notes
The Snca snca (Catalog #AAA27709) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Alpha Synuclein Antibody (Aggregate-Specific) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Alpha Synuclein Antibody (Aggregate-Specific) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Dot Blot (DB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), ImmunoGold labelling. WB: 1:1000. Researchers should empirically determine the suitability of the Snca snca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Alpha Synuclein Antibody (Aggregate-Specific), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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