WB (Western Blot)
(alpha Tubulin mAb (Clone 5-B-1-2) tested by Western blot. HeLa whole-cell extract (15 ug/lane) probed with alpha Tubulin mAb (1:5,000 dilution).)
Mouse alpha Tubulin Monoclonal Antibody
alpha Tubulin antibody (mAb)(Clone 5-B-1-2)
Reactivity
Human, Mouse, Wide Range Predicted
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
Ascites
Synonyms
alpha Tubulin, Antibody; alpha Tubulin antibody (mAb)(Clone 5-B-1-2); anti-alpha Tubulin antibody
Host
Mouse
Reactivity
Human, Mouse, Wide Range Predicted
Clonality
Monoclonal
Isotype
IgG1
Clone Number
5-B-1-2
Purity/Purification
Ascites
Form/Format
Ascites containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-alpha Tubulin antibody
WB (Western Blot), IF (Immunofluorescence), ICC (Immunocytochemistry)
Immunogen
This alpha Tubulin antibody was raised against total protein purified from sea urchin sperm axonemes.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
WB (Western Blot)
(alpha Tubulin mAb (Clone 5-B-1-2) tested by Western blot. HeLa whole-cell extract (15 ug/lane) probed with alpha Tubulin mAb (1:5,000 dilution).)
WB (Western Blot)
(alpha Tubulin mAb (Clone 5-B-1-2) tested by Western blot. HeLa whole-cell extract (15 ug/lane) probed with alpha Tubulin mAb (1:5,000 dilution).)
IF (Immunofluorescence)
(alpha Tubulin mAb (Clone 5-B-1-2) tested by immunofluorescence. Top: HeLa cells stained with alpha Tubulin mAb at 1:500 dilution (red). Middle: Same cells stained with DAPI (blue). Bottom: Both images merged. Staining was carried out using MAX Stain Immunofluorescence Tools.)
IF (Immunofluorescence)
(alpha Tubulin mAb (Clone 5-B-1-2) tested by immunofluorescence. Top: HeLa cells stained with alpha Tubulin mAb at 1:500 dilution (red). Middle: Same cells stained with DAPI (blue). Bottom: Both images merged. Staining was carried out using MAX Stain Immunofluorescence Tools.)
Related Product Information for anti-alpha Tubulin antibody
Short Description: Our alpha Tubulin antibody (mAb)(Clone 5-B-1-2) was raised in a Mouse host. It has been validated for use in Immunocytochemistry, Immunofluorescence and Western blot; it has been shown to react with Human and Mouse samples, but it is predicted that it will react with a wide range of sample types.
Background: Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of a-tubulin and b-tubulin. Alpha Tubulin expression is reduced in cells deficient in MeCP2, leading to conditions such as Rett syndrome and autistic-spectrum disorders.
Background: Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of a-tubulin and b-tubulin. Alpha Tubulin expression is reduced in cells deficient in MeCP2, leading to conditions such as Rett syndrome and autistic-spectrum disorders.
Product Categories/Family for anti-alpha Tubulin antibody
NCBI and Uniprot Product Information
Molecular Weight
52kDa
Similar Products
Product Notes
The alpha Tubulin (Catalog #AAA59873) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The alpha Tubulin antibody (mAb)(Clone 5-B-1-2) reacts with Human, Mouse, Wide Range Predicted and may cross-react with other species as described in the data sheet. AAA Biotech's alpha Tubulin can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), ICC (Immunocytochemistry). Researchers should empirically determine the suitability of the alpha Tubulin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "alpha Tubulin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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