Mouse anti-Human alpha-V/beta-3 Integrin Monoclonal Antibody

alpha-V/beta-3 Integrin, Human, mAb BV3

Cat. Num. AAA77790

• Reactivity: Human
• Applications: Immunoprecipitation, Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
• Purity: Protein G
• Synonyms: alpha-V/beta-3 Integrin, Antibody; alpha-V/beta-3 Integrin, Human, mAb BV3; Vitronectin receptor, integrin alpha-v/beta-3, cd51/cd61; anti-alpha-V/beta-3 Integrin antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Purity/Purification: Protein G
• Form/Format: 0.2 Um filtered in PBS + 0.1% BSA + 0.02% NaN3
• Concentration: 100 ug/ml (varies by lot)

• Applicable Applications for anti-alpha-V/beta-3 Integrin antibody: IP (Immunoprecipitation), IA (Immunoassay), FCM/FACS (Flow Cytometry), IF (Immunofluorescence), IHC (Immunohistochemistry)
• Cross-Reactivity: Chicken, Rat
• Immunogen: Full length human alpha-V/beta-3 Integrin protein
• Preparation and Storage: Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.

FCM/FACS (Flow Cytometry)

(FC: HUVEC cells were incubated with 2 ug/ml AAA77790 for 1h at 4°C. Black: isotype control mouse IgG1, Red: cells only, Blue: AAA77790, clone BV3.)

Related Product Information for anti-alpha-V/beta-3 Integrin antibody

The monoclonal antibody BV3 recognizes human alpha-V/beta-3 integrin present on human cells. Integrins are a superfamily of alphabeta heterodimeric cell-surface adhesion receptors found in many species. They are expressed on a variety of cells and mediate numerous physiological processes, including inflammation, migration, adhesion and proliferation. The β3 family consist of 2 members: αIIbβ3 and αvβ3, which mediate cell-cell and cell-ECM interactions and are important for cellular migration, regulation of gene expression, cell survival, adhesion and differentiation. All processes which are involved in tissue development, angiogenesis and thrombosis. Each subunit consist of an extracellular domain, a single transmembrane segment and a cytoplasmic tail. They connect to the actin cytoskeleton via adaptor proteins that bind theircytoplasmic tails. Cell matrix adhesions also act as signaling units by their capacity to organize the actin cytoskeleton and to accumulate various signaling intermediates. Integrin alphavbeta3 was originally identified as the vitronectin receptor. Nevertheless, other ligands include fibrinogen, fibronectin, laminin, thrombospondin, Von Willebrand factor, tenascin, osteopontin and several forms of collagen. The interactions of integrin αvβ3 to those ligands is mediated by the RGD (Arg-Gly-Asp) sequence motif present in these proteins. Deregulation of β3 integrins is involved in e.g. autoimmune diseases, cardiovascular disorders, transplant rejection and tumorigenesis. In contribution to the latter, integrin αvβ3 contribute by supporting growth of small (tumor) blood vessels thereby potentiating the metastatic potential. Overexpression of integrin αvβ3 has been demonstrated in various tumors and activated endothelium.

References

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2. Merkel, O et al.; Integrin alphavbeta3 targeted gene delivery using RGD peptidomimetic conjugates with copolymers of PRGylated poly(ethyleneimine). Bioconj chem.. 2009, 20:1270-1280
3. Dare, E et al.; Fibrin sealants from fresh/frozen plasma as scaffolds for in vitro articular cartilage regeneration. Tissue engineering, 2009, 15:2285