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IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Breast cancer using AMPK beta 1 Mouse mAb (1/200 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).)
Mouse AMPK beta 1 Monoclonal Antibody | anti-AMPK beta 1 antibody
AMPK beta 1 Monoclonal Antibody
Gene Names
PRKAB1; AMPK; HAMPKb
Reactivity
Human, Mouse, Rat, Monkey
Applications
Immunohistochemistry, Immunoprecipitation, Immunocytochemistry, Western Blot
Purity
Affinity purified
Synonyms
AMPK beta 1, Antibody; AMPK beta 1 Monoclonal Antibody; 1300015D22Rik; 5 AMP activated protein kinase subunit beta 1; 5''-AMP-activated protein kinase subunit beta-1; AAKB1_HUMAN; AMP-ACTIVATED PROTEIN KINASE, NONCATALYTIC, BETA-1; AMP-activated, noncatalytic, beta-1; AMPK; AMPK beta 1 chain; anti-AMPK beta 1 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat, Monkey
Clonality
Monoclonal
Clone Number
1A7-E11-E9
Specificity
This antibody detects endogenous levels of AMPK beta 1 and does not cross-react with related proteins.
Purity/Purification
Affinity purified
Form/Format
Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
Sequence Length
270
Applicable Applications for anti-AMPK beta 1 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), ICC (Immunocytochemistry), WB (Western Blot)
Immunogen Type
Recombinant Protein
Immunogen Description
Purified recombinant human AMPK beta 1 protein fragments expressed in E Coli.
Target Name
AMPK beta 1
Preparation and Storage
Store at -20 degree C
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Breast cancer using AMPK beta 1 Mouse mAb (1/200 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Breast cancer using AMPK beta 1 Mouse mAb (1/200 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of Hela cell lysates using AMPK beta 1 antibody.)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of Hela cell lysates using AMPK beta 1 antibody.)
WB (Western Blot)
(Western blot detection of AMPK beta 1 in 3T3,Hela,PC-12,COS7 and MDA-MB-468 cell lysates using AMPK beta 1 antibody (1:1000 diluted).Predicted band size:38KDa.Observed band size:38KDa.Exposure time:5min.)
WB (Western Blot)
(Western blot detection of AMPK beta 1 in 3T3,Hela,PC-12,COS7 and MDA-MB-468 cell lysates using AMPK beta 1 antibody (1:1000 diluted).Predicted band size:38KDa.Observed band size:38KDa.Exposure time:5min.)
ICC (Immunocytochemistry)
(Immunocytochemistry staining of HeLa cells fixed with 1% Paraformaldehyde and using anti-AMPK beta 1 antibody (dilution 1:100).)
ICC (Immunocytochemistry)
(Immunocytochemistry staining of HeLa cells fixed with 1% Paraformaldehyde and using anti-AMPK beta 1 antibody (dilution 1:100).)
Related Product Information for anti-AMPK beta 1 antibody
Non-catalytic subunit of AMP-activated protein kinase (AMPK),an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism.In response to reduction of intracellular ATP levels,AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein,carbohydrate and lipid biosynthesis,as well as cell growth and proliferation.AMPK acts via direct phosphorylation of metabolic enzymes,and by longer-term effects via phosphorylation of transcription regulators.Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton;probably by indirectly activating myosin.Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles,via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1,PRKAG2 or PRKAG3).
Product Categories/Family for anti-AMPK beta 1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,382 Da
NCBI Official Full Name
5'-AMP-activated protein kinase subunit beta-1
NCBI Official Synonym Full Names
protein kinase, AMP-activated, beta 1 non-catalytic subunit
NCBI Official Symbol
PRKAB1
NCBI Official Synonym Symbols
AMPK; HAMPKb
NCBI Protein Information
5'-AMP-activated protein kinase subunit beta-1
UniProt Protein Name
5'-AMP-activated protein kinase subunit beta-1
UniProt Gene Name
PRKAB1
UniProt Synonym Gene Names
AMPK; AMPK subunit beta-1; AMPKb
UniProt Entry Name
AAKB1_HUMAN
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Product Notes
The AMPK beta 1 prkab1 (Catalog #AAA300758) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AMPK beta 1 Monoclonal Antibody reacts with Human, Mouse, Rat, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's AMPK beta 1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the AMPK beta 1 prkab1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AMPK beta 1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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