Mouse anti-Human Annexin A1 Monoclonal Antibody | anti-ANXA1 antibody

Annexin A1/(Hairy Cell Leukemia Marker)

Cat. Num. AAA215010

• Gene Names: ANXA1; ANX1; LPC1
• Reactivity: Human
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Annexin A1, Antibody; Annexin A1/(Hairy Cell Leukemia Marker); ANXA1; ANX1; Annexin-1; Chromobindin-9; LPC1; p35; anti-ANXA1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone Number: ANXA1/1671
• Specificity: The ANXA1 gene belongs to the annexin family, and contains 4 annexin repeats. A pair of annexin repeats may form one binding site for calcium and a phospholipid. ANXA1 promotes membrane fusion and is involved in exocytosis. The gene for ANXA1 is upregulated in hairy cell leukemia (HCL), and its protein expression is specific for HCL. Detection of ANXA1 provides a simple, highly sensitive and specific assay for HCL. Annexin A1 has also been found to be protective against DNA damage induced by heat in breast cancer cells, suggesting it is involved in tumor suppressive and protective activities, and also is associated with treatment resistance.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 346

• Applicable Applications for anti-ANXA1 antibody: IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry)
• Positive Control: A431 and K562 cell lysates, human esophagus, placenta and prostate tissues.
• Immunogen: Recombinant full length human Annexin A1 protein
• Cellular Localization: Nucleus. Cytoplasm. Cell projection > cilium. Basolateral Cell Membrane. Found in the cilium, nucleus and basolateral Cell Membrane of ciliated cells in the tracheal endothelium, also found in the cyt
• Hu-chromosome Location: 9q21.13
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

FCM/FACS (Flow Cytometry)

(Flow Cytometric Analysis of PFA-fixed HeLa cells. Annexin A1 Mouse Monoclonal Antibody (ANXA1/1671) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)

SDS-PAGE

(SDS-PAGE Analysis Purified Annexin A1 Mouse Monoclonal Antibody (ANXA1/1671). Confirmation of Integrity and Purity of Antibody.)

IHC (Immunohiostchemistry)

(Formalin-fixed, paraffin-embedded human Prostate tissue stained with Annexin A1 Mouse Monoclonal Antibody (ANXA1/1671).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Spleen tissue stained with Annexin A1 Mouse Monoclonal Antibody (ANXA1/1671).)

References

1. Basso K, Liso A, Tiacci E et al. J Exp Med 199:59-68, 2004. 2. Falini B, Tiacci E, Liso A et al. Lancet 363: 1869-1871, Jun 5, 2004.

NCBI and Uniprot Product Information

• NCBI GI #: 4502101
• NCBI GeneID: 301
• NCBI Accession #: NP_000691.1
• NCBI GenBank Nucleotide #: NP_000691.1
• UniProt Accession #: P04083
• Molecular Weight: 35kDa
• NCBI Official Full Name: annexin A1
• NCBI Official Synonym Full Names: annexin A1
• NCBI Official Symbol: ANXA1
• NCBI Official Synonym Symbols: ANX1; LPC1
• NCBI Protein Information: annexin A1
• UniProt Protein Name: Annexin A1
• UniProt Gene Name: ANXA1
• UniProt Synonym Gene Names: ANX1; LPC1
• UniProt Entry Name: ANXA1_HUMAN