Mouse anti-Human ANPEP/CD13 Monoclonal Antibody | anti-ANPEP antibody

ANPEP/CD13 Mouse anti-Human Monoclonal (aa668-967)(2F10) Antibody

Cat. Num. AAA162712

• Gene Names: ANPEP; APN; CD13; LAP1; P150; PEPN; GP150
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunohistochemistry
• Purity: Immunoaffinity purified
• Synonyms: ANPEP/CD13, Antibody; ANPEP/CD13 Mouse anti-Human Monoclonal (aa668-967)(2F10) Antibody; PathPlus ANPEP/CD13 Antibody (aa668-967; clone 2F10); ANPEP; Alanyl aminopeptidase; Aminopeptidase M; APN; Aminopeptidase N; AP-N; AP-M; CD13; CD13 antigen; HAPN; gp150; p150; PEPN; Microsomal aminopeptidase; LAP1; anti-ANPEP antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 2F10
• Specificity: Human ANPEP/CD13
• Purity/Purification: Immunoaffinity purified
• Form/Format: PBS, pH7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-ANPEP antibody: WB (Western Blot), IHC (Immunohistochemistry), IHC (Immunohistochemistry)
• Target: Human ANPEP/CD13
• Immunogen: Human recombinant protein fragment corresponding to amino acids 668-967 of human ANPEP (NP_001141) produced in E Coli.
• Conjugation: Unconjugated
• Epitope: aa668-967
• Family: Exopeptidase
• Subfamily: Metallopeptidase M1
• Preparation and Storage: Store at -20 degree C. Avoid freeze-thaw cycles.

IHC (Immunohistochemistry)

(ANPEP/CD13 Antibody-Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemisry)

(ANPEP/CD13 Antibody-Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohiostchemistry)

(ANPEP/CD13 Antibody-Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE))

WB (Western Blot)

(ANPEP/CD13 Antibody-HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANPEP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANPEP (1:2000).)

Related Product Information for anti-ANPEP antibody

CD13 antibody is an unconjugated mouse monoclonal antibody to human CD13 (ANPEP)(aa668-967). Validated for IHC and WB.
ANPEP (also known as CD13, AAP, AP-N, LAP1, and P150) is a broad specificity alanyl aminopeptidase involved in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. It is thought to function in the metabolism of regulatory peptides of diverse cell types, responsible for the processing of peptide hormones (angiotensin III and IV), neuropeptides, and chemokines. It is thought to play an important role in the pathogenesis of cholesterol gallstone disease. ANPEP also cleaves antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and serves to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. It is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. It is also a useful and specific marker for mature zymogenic chief cells (ZCs) present in gastric epithelium, and expression on ZCs is lost in transition to metaplasia in the stomach. In immunohistochemistry of normal tissue, ANPEP has membranous and cytoplasmic positivity in the prostate, liver, gallbladder, exocrine pancreas, intestine and renal tubules.

References

The UniProt Consortium. Nucleic Acids Res. 47: D506-515 (2019); Nucleic Acids Res. 2016 Jan 4;44(D1):D733-45, PMID:26553804; Am J Physiol Gastrointest Liver Physiol. 2015 Dec 15; 309(12): G955 -G964, PMID: 26514774;

NCBI and Uniprot Product Information

• NCBI GI #: 157266300
• NCBI GeneID: 290
• NCBI Accession #: NP_001141.2
• NCBI GenBank Nucleotide #: NM_001150
• UniProt Accession #: P15144; Q16728; Q6GT90; Q8IUK3; Q8IVH3; Q9UCE0
• Molecular Weight: 109,540 Da
• NCBI Official Full Name: aminopeptidase N
• NCBI Official Synonym Full Names: alanyl (membrane) aminopeptidase
• NCBI Official Symbol: ANPEP
• NCBI Official Synonym Symbols: APN; CD13; LAP1; P150; PEPN; GP150
• NCBI Protein Information: aminopeptidase N; AP-M; AP-N; hAPN; aminopeptidase M; alanyl aminopeptidase; microsomal aminopeptidase; myeloid plasma membrane glycoprotein CD13
• UniProt Protein Name: Aminopeptidase N
• UniProt Gene Name: ANPEP
• UniProt Synonym Gene Names: APN; CD13; PEPN; AP-N; hAPN; AP-M
• UniProt Entry Name: AMPN_HUMAN