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ICC (Immunocytochemistry)
(ICC staining Apaf-1 in Ags cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Rabbit anti-Human, Mouse Apaf-1 Monoclonal Antibody | anti-Apaf-1 antibody
Apaf-1 Antibody
Gene Names
APAF1; CED4; APAF-1
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
Purity
ProA affinity purified
Synonyms
Apaf-1, Antibody; Apaf-1 Antibody; APAF 1 antibody; Apaf-1 antibody; APAF_HUMAN antibody; Apaf1 antibody; Apoptotic peptidase activating factor 1 antibody; Apoptotic protease activating factor 1 antibody; Apoptotic protease activating factor antibody; Apoptotic protease-activating factor 1 antibody; CED 4 antibody; CED4 antibody; KIAA0413 antibody; anti-Apaf-1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Monoclonal
Clone Number
SY22-02
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-Apaf-1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes
WB: 1:1000
IHC: 1:50-1:200
ICC: 1:50-1:200
IHC: 1:50-1:200
ICC: 1:50-1:200
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
ICC (Immunocytochemistry)
(ICC staining Apaf-1 in Ags cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Apaf-1 in Ags cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Apaf-1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Apaf-1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Apaf-1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Apaf-1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Apaf-1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Apaf-1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-Apaf-1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-Apaf-1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Apaf-1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Apaf-1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Apaf-1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Apaf-1 antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of Apaf-1 on HUVEC cell lysates using anti-Apaf-1 antibody at 1/1, 000 dilution.)
WB (Western Blot)
(Western blot analysis of Apaf-1 on HUVEC cell lysates using anti-Apaf-1 antibody at 1/1, 000 dilution.)
Related Product Information for anti-Apaf-1 antibody
The mammalian homologs of the Ced-4 proteins, Apaf-1 (Ced-4), Nod1 (CARD4), and Nod2 contain a caspase recruitment domain (CARD) and a putative nucleotide binding domain, signified by a consensus Walker's A box (P-loop) and B box (Mg2+-binding site). Nod1 contains a putative regulatory domain and multiple leucine-rich repeats. Nod1 is a member of a growing family of intracellular proteins which share structural homology to the apoptosis regulator Apaf-1. Nod1 associates with the CARD-containing kinase RICK and activates NFkB. The self-association of Nod1 mediates proximity of RICK and the interaction of RICK with IKKg. In addition, Nod-1 binds to multiple caspases with long prodomains, but specifically activates caspase-9 and promotes caspase-9-induced apoptosis. Nod2 is composed of two N-terminal CARDs, a nucleotide-binding domain, and multiple C-terminal leucine-rich repeats. The expression of Nod2 is highly restricted to monocytes, and activates NFkB in response to bacterial lipopoly-saccharides.
Product Categories/Family for anti-Apaf-1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
141,840 Da
NCBI Official Full Name
apoptotic protease-activating factor 1 isoform b
NCBI Official Synonym Full Names
apoptotic peptidase activating factor 1
NCBI Official Symbol
APAF1
NCBI Official Synonym Symbols
CED4; APAF-1
NCBI Protein Information
apoptotic protease-activating factor 1
UniProt Protein Name
Apoptotic protease-activating factor 1
UniProt Gene Name
APAF1
UniProt Synonym Gene Names
KIAA0413; APAF-1
UniProt Entry Name
APAF_HUMAN
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Product Notes
The Apaf-1 apaf1 (Catalog #AAA30052) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Apaf-1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Apaf-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC). WB: 1:1000 IHC: 1:50-1:200 ICC: 1:50-1:200. Researchers should empirically determine the suitability of the Apaf-1 apaf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Apaf-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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