IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using APAF-1-ALT antibody.)
Rabbit anti-Human APAF-1-ALT Polyclonal Antibody | anti-APAF-1-ALT antibody
APAF-1-ALT Antibody
Gene Names
APAF1; CED4; APAF-1
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
APAF-1-ALT, Antibody; APAF-1-ALT Antibody; APAF1-interacting protein; APIP; APIP2; CGI-29 protein; MMRP19; anti-APAF-1-ALT antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total APAF-1-ALT protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
1194
Applicable Applications for anti-APAF-1-ALT antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen Type
Peptide
Immunogen Description
Synthesized peptide derived from human APAF-1-ALT.
Target Name
APAF-1-ALT
Preparation and Storage
Store at -20 degree C
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using APAF-1-ALT antibody.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using APAF-1-ALT antibody.)
WB (Western Blot)
(Western blot analysis of extracts from COLO205, using APAF-1-ALT antibody.)
WB (Western Blot)
(Western blot analysis of extracts from COLO205, using APAF-1-ALT antibody.)
Related Product Information for anti-APAF-1-ALT antibody
This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.Keigo Machida, J. Biol. Chem., Apr 2001; 276: 12140 - 12146.Liam O¡¯Connor, Cancer Res., Mar 2000; 60: 1217 - 1220.Zeping Wang, Nucleic Acids Res., Apr 2005; 33: 2248 - 2258Govindaraj Anumanthan, Cancer Res., Nov 2006; 66: 10824 - 10832.
Product Categories/Family for anti-APAF-1-ALT antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
37,976 Da
NCBI Official Full Name
apoptotic protease-activating factor 1 isoform b
NCBI Official Synonym Full Names
apoptotic peptidase activating factor 1
NCBI Official Symbol
APAF1
NCBI Official Synonym Symbols
CED4; APAF-1
NCBI Protein Information
apoptotic protease-activating factor 1
UniProt Protein Name
Apoptotic protease-activating factor 1
UniProt Gene Name
APAF1
UniProt Synonym Gene Names
KIAA0413; APAF-1
UniProt Entry Name
APAF_HUMAN
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Product Notes
The APAF-1-ALT apaf1 (Catalog #AAA303795) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The APAF-1-ALT Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's APAF-1-ALT can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the APAF-1-ALT apaf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "APAF-1-ALT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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