IHC (Immunohiostchemistry)
(Immunohistochemistry of Apaf1 in K562 cells with Apaf1 antibody at 0.5 ug/mL.)
Mouse Apaf-1 Monoclonal Antibody | anti-Apaf-1 antibody
Apaf-1 Monoclonal Antibody
Gene Names
APAF1; CED4; APAF-1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Immunoaffinity chromotography purified IgG
Synonyms
Apaf-1, Antibody; Apaf-1 Monoclonal Antibody; Apaf-1 (2E10), Apoptotic protease activating factor 1; anti-Apaf-1 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
2E10
Purity/Purification
Immunoaffinity chromotography purified IgG
Form/Format
Supplied in PBS containing 0.02% sodium azide.
Sequence Length
1194
Applicable Applications for anti-Apaf-1 antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA
Immunogen Type
Peptide
Immunogen Description
A peptide corresponding to amino acids near the carboxy terminus of human Apaf1.
Target Name
Apaf-1
Preparation and Storage
Can be stored at -20 degree C, stable for one year.
IHC (Immunohiostchemistry)
(Immunohistochemistry of Apaf1 in K562 cells with Apaf1 antibody at 0.5 ug/mL.)
IHC (Immunohiostchemistry)
(Immunohistochemistry of Apaf1 in K562 cells with Apaf1 antibody at 0.5 ug/mL.)
WB (Western Blot)
(Western blot analysis of Apaf1 in K562 cell lysate with Apaf1 antibody at (A) 0.25 and (B) 0.5 mg/ml.)
WB (Western Blot)
(Western blot analysis of Apaf1 in K562 cell lysate with Apaf1 antibody at (A) 0.25 and (B) 0.5 mg/ml.)
Related Product Information for anti-Apaf-1 antibody
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologous of the key cell death gene CED-4 in C. elegans was identified recently from human and mouse and designated Apaf1 for apoptosis protease-activating factor 1 (1,2). Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Apaf1 transcript is ubiquitously expressed in human tissues.
Product Categories/Family for anti-Apaf-1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
37,976 Da
NCBI Official Full Name
apoptotic protease activating factor 1
NCBI Official Synonym Full Names
apoptotic peptidase activating factor 1
NCBI Official Symbol
APAF1
NCBI Official Synonym Symbols
CED4; APAF-1
NCBI Protein Information
apoptotic protease-activating factor 1
UniProt Protein Name
Apoptotic protease-activating factor 1
UniProt Gene Name
APAF1
UniProt Synonym Gene Names
KIAA0413; APAF-1
UniProt Entry Name
APAF_HUMAN
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Product Notes
The Apaf-1 apaf1 (Catalog #AAA300855) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Apaf-1 Monoclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Apaf-1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the Apaf-1 apaf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Apaf-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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