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IP (Immunoprecipitation)
(AGO2 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg MCF-7 Whole Cell LysateLane C:0.5 mg HEK293 Whole Cell Lysate4 uL anti-AGO2 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-AGO2 rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 100 kDaObserved band size :100 kDa)
Rabbit anti-Mouse Argonaute-2 Monoclonal Antibody | anti-AGO2 antibody
Recombinant Anti-Argonaute-2 Antibody, Rabbit Monoclonal
Gene Names
Ago2; Eif2c2; Gerp95; Gm10365; AI225898; AL022874; AW546247; mKIAA4215; 1110029L17Rik; 2310051F07Rik; ENSMUSG00000072493
Reactivity
Mouse
Applications
Immunoprecipitation, Immunohistochemistry, ELISA
Purity
Protein A
Synonyms
Argonaute-2, Antibody; Recombinant Anti-Argonaute-2 Antibody, Rabbit Monoclonal; AGO2/Argonaute 2/EIF2C2 Antibody, Rabbit MAb; argonaute RISC catalytic component 2; Anti-1110029L17Rik Antibody; Anti-2310051F07Rik Antibody; Anti-Ago2 Antibody; Anti-AI225898 Antibody; Anti-AL022874 Antibody; Anti-AW546247 Antibody; Anti-Eif2c2 Antibody; Anti-ENSMUSG00000072493 Antibody; Anti-Gerp95 Antibody; Anti-Gm10365 Antibody; Anti-KIAA4215 Antibody; Anti-mKIAA4215 Antibody; anti-AGO2 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
036
Specificity
Mouse Argonaute-2
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-AGO2 antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Mouse AGO2 protein
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse AGO2 (rM AGO2).
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(AGO2 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg MCF-7 Whole Cell LysateLane C:0.5 mg HEK293 Whole Cell Lysate4 uL anti-AGO2 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-AGO2 rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 100 kDaObserved band size :100 kDa)
IP (Immunoprecipitation)
(AGO2 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg MCF-7 Whole Cell LysateLane C:0.5 mg HEK293 Whole Cell Lysate4 uL anti-AGO2 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-AGO2 rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 100 kDaObserved band size :100 kDa)
IHC (Immunohistochemisry)
(Immunochemical staining of AGO2 in human brain with rabbit monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemisry)
(Immunochemical staining of AGO2 in human brain with rabbit monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)
IP (Immunoprecipitation)
(AGO2 was immunoprecipitated using:Lane A:0.5 mg K562 Whole Cell LysateLane B:0.5 mg Raw264.7 Whole Cell Lysate2 uL anti-Mouse AGO2 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse AGO2 rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 100 kDaObserved band size: 100 kDa)
IP (Immunoprecipitation)
(AGO2 was immunoprecipitated using:Lane A:0.5 mg K562 Whole Cell LysateLane B:0.5 mg Raw264.7 Whole Cell Lysate2 uL anti-Mouse AGO2 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse AGO2 rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 100 kDaObserved band size: 100 kDa)
IHC (Immunohistochemistry)
(Immunochemical staining of AGO2 in mouse brain with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).)
IHC (Immunohistochemistry)
(Immunochemical staining of AGO2 in mouse brain with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).)
Related Product Information for anti-AGO2 antibody
Argonaute 2 (AGO2), also known as Eukaryotic translation initiation factor 2C2 (EIF2C2), belongs to the Argonaute family, AGO subfamily, which is a component of the RNA-induced silencing complex (RISC) and mediates small interfering RNA (siRNA)-directed mRNA cleavage and microRNA translational suppression. AGO2 protein is the catalytic engine of mammalian RNAi. It contains a PIWI domain that is structurally related to RNases H and possibly shares with them a two-metal-ion catalysis mechanism. Human AGO2 was unable to cleave preformed RNA duplexes and exhibited weaker binding affinity for RNA duplexes compared with the single strand RNA. The enzyme exhibited greater RNase H activity in the presence of Mn2+ compared with Mg2+. Human AGO2 exhibited weaker binding affinities and reduced cleavage activities for antisense RNAs with either a 5'-terminal hydroxyl or abasic nucleotide. In mouse hematopoiesis, AGO2 controls early development of lymphoid and erythroid cells. AGO2 is a highly specialized member of the Argonaute family with an essential nonredundant Slicer-independent function within the mammalian miRNA pathway. AGO2 regulates dFMR1 expression, and the relationship between dFMR1 and AGO2 was defined by their physical interaction and co-regulation of downstream targets. AGO2 and dFMR1 are also connected through a regulatory relationship. AGO2 is a regulator of dFMR1 expression and have clarified an important developmental role for AGO2 in the nervous system and germ line that requires dFMR1 function. In addition, AGO2 is regulated at both the transcriptional and posttranslational level, and also implicate AGO2 and enhanced micro-RNA activity in the tumorigenic progression of breast cancer cell lines.
References
O'Carroll D, et al. (2007) A Slicer-independent role for Argonaute 2 in hematopoiesis and the microRNA pathway. Genes Dev. 21(16): 1999-2004.Pepper AS, et al. (2009) Argonaute2 suppresses Drosophila fragile X expression preventing neurogenesis and oogenesis defects. PLoS One. 4(10): e7618.Lima WF, et al. (2009) Binding and cleavage specificities of human Argonaute2. J Biol Chem. 284(38): 26017-28.Adams BD, et al. (2009) Argonaute-2 expression is regulated by epidermal growth factor receptor and mitogen-activated protein kinase signaling and correlates with a transformed phenotype in breast cancer cells. Endocrinology. 150(1): 14-23.Salvatore V, et al. (2010) Bacterial expression of mouse argonaute 2 for functional and mutational studies. Int J Mol Sci. 11(2): 745-53.Wilson JA, et al. (2011) Human Ago2 is required for efficient microRNA 122 regulation of hepatitis C virus RNA accumulation and translation. J Virol. 85(5): 2342-50.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
97,304 Da
NCBI Official Full Name
protein argonaute-2
NCBI Official Synonym Full Names
argonaute RISC catalytic subunit 2
NCBI Official Symbol
Ago2
NCBI Official Synonym Symbols
Eif2c2; Gerp95; Gm10365; AI225898; AL022874; AW546247; mKIAA4215; 1110029L17Rik; 2310051F07Rik; ENSMUSG00000072493
NCBI Protein Information
protein argonaute-2
UniProt Protein Name
Protein argonaute-2
UniProt Gene Name
Ago2
UniProt Synonym Gene Names
Eif2c2; Kiaa4215; Argonaute2; mAgo2; eIF-2C 2; eIF2C 2
UniProt Entry Name
AGO2_MOUSE
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Product Notes
The AGO2 ago2 (Catalog #AAA257206) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-Argonaute-2 Antibody, Rabbit Monoclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Argonaute-2 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the AGO2 ago2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Argonaute-2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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