Rabbit anti-Human, Mouse Protein argonaute-2 Polyclonal Antibody | anti-AGO2 antibody

Rabbit anti-human Protein argonaute-2 polyclonal Antibody

Cat. Num. AAA118544

• Gene Names: AGO2; Q10; EIF2C2
• Reactivity: Human, Mouse
• Applications: Chromatin Immunoprecipitation, Immunoprecipitation, Immunohistochemistry, Western Blot, ELISA
• Purity: >95%, Protein G purified
• Synonyms: Protein argonaute-2, Antibody; Rabbit anti-human Protein argonaute-2 polyclonal Antibody; Argonaute RISC catalytic component 2; Eukaryotic translation initiation factor 2C 2; PAZ Piwi domain protein; Protein slicer; EIF2C2; AGO2; anti-AGO2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid

• Applicable Applications for anti-AGO2 antibody: ChIP (Chromatin immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot), ELISA
• Immunogen: Recombinant Human Protein argonaute-2 protein (517-818AA)
• Conjugate: Non-conjugated
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Species: Homo sapiens (Human)
• Research Area: Epigenetics and Nuclear Signaling
• Target Names: AGO2
• Preparation and Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

IHC (Immunohistochemistry)

(IHC image of AAA118544 diluted at 1:1200 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human kidney tissue using AAA118544 at dilution of 1:100)

ChIP (Chromatin Immunoprecipitation)

(Chromatin Immunoprecipitation Hela(1.1^106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-AgO2 or a control normal rabbit IgG . The resulting ChIP DNA was quantified using real-time PCR with primers against the SLC1A5 promoter.)

IHC (Immunohiostchemistry)

(IHC image of AAA118544 diluted at 1:1200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

WB (Western Blot)

(Western blotAll lanes: Protein argonaute-2 antibody at 2ug/mlLane 1: A549 whole cell lysateLane 2: Jurkats whole cell lysateLane 3: MCF-7 whole cell lysateLane 4: HepG2 whole cell lysateLane 5: Raw264.7 whole cell lysateLane 6: K562 whole cell lysateLane 7: Mouse liver tissueLane 8: Mouse kidney tissueGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 98,94 kDaObserved band size: 90 kDa)

Related Product Information for anti-AGO2 antibody

Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The minimal RISC appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7- methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also up-regulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3-UTR of the TNF (TNF-alpha) mRNA and upregulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions.

NCBI and Uniprot Product Information

• NCBI GI #: 257467482
• NCBI GeneID: 27161
• NCBI Accession #: NP_001158095.1
• NCBI GenBank Nucleotide #: NM_001164623.1
• UniProt Accession #: Q9UKV8; Q8TCZ5; Q8WV58; Q96ID1
• NCBI Official Full Name: protein argonaute-2 isoform 2
• NCBI Official Synonym Full Names: argonaute 2, RISC catalytic component
• NCBI Official Symbol: AGO2
• NCBI Official Synonym Symbols: Q10; EIF2C2
• NCBI Protein Information: protein argonaute-2
• UniProt Protein Name: Protein argonaute-2
• UniProt Gene Name: AGO2
• UniProt Synonym Gene Names: EIF2C2; Argonaute2; hAgo2; eIF-2C 2; eIF2C 2; PPD
• UniProt Entry Name: AGO2_HUMAN