Rabbit anti-Human ATP1B4 Monoclonal Antibody | anti-ATP1B4 antibody

Recombinant Anti-ATP1B4 Antibody, Rabbit Monoclonal

Cat. Num. AAA255622

• Reactivity: Human
• Applications: Immunoprecipitation, ELISA, Western Blot
• Purity: Protein A
• Synonyms: ATP1B4, Antibody; Recombinant Anti-ATP1B4 Antibody, Rabbit Monoclonal; ATP1B4 Antibody, Rabbit MAb; ATPase, Na+/K+ transporting, beta 4 polypeptide; anti-ATP1B4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 108
• Specificity: Human ATP1B4
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-ATP1B4 antibody: IP (Immunoprecipitation), ELISA, WB (Western Blot)
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Human ATP1B4 protein
• Conjugation: Unconjugated
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human ATP1B4 (mature form, NP_036201.1; Tyr 132-Thr 357).
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

IP (Immunoprecipitation)

(ATP1B4 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate0.5 uL anti-ATP1B4 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ATP1B4 rabbit monoclonal antibody,at 1:1000 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 40 kDaObserved band size: 40 kDa)

WB (Western Blot)

(Anti-ATP1B4 rabbit monoclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:42 kDaObserved band size:38 kDa)

Related Product Information for anti-ATP1B4 antibody

ATP1B4 is a member of the X(+)/potassium ATPases subunit beta family. It is highly expressed in skeletal muscle and at a lower level in heart. ATP1B4 gene can be found in all vertebrate genomes sequenced to date. However, this gene has undergone a change in function in placental mammals compared to other species. Specifically, in fish, avian, and amphibian species, this gene encodes plasma membrane-bound beta-subunits of Na, K-ATPase. In placental mammals, the encoded protein interacts with the nuclear transcriptional coregulator SKIP and may be involved in the regulation of TGF-beta signaling. ATP1B4 may act as a transcriptional coregulator during muscle development through its interaction with SNW1. Na+, K+-ATPase is an important regulator of intracellular electrolyte levels in most mammalian cells. It is a Mg2+-dependent transport pump responsible for maintaining the low intracellular Na+:K+ ratio that is essential for cell homeostasis and physiological function. It catalyzes the active uptake of K+ and extrusion of Na+ at the expense of hydrolyzing ATP with a stoichiometry of 3 Na+ for 2 K+. ATP1B4 has lost its ancestral function as a Na, K-ATPase beta-subunit.

References

Pestov NB. et al., 2007, Proc Natl Acad Sci. 104 (27): 11215-20.Kung AW. et al., 2006, Clin Endocrinol. 64 (2): 158-61.Gerhard DS. et al., 2004, Genome Res. 14 (10B): 2121-7.