Standard Curve (Sample)
Mouse ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4) ELISA Kit | ATP1b4 elisa kit
Mouse ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4) ELISA Kit
Gene Names
Atp1b4; 1110020B02Rik
Reactivity
Mouse
Synonyms
ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4); N/A; Mouse ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4) ELISA Kit; X,K-ATPase subunit beta-m; X/potassium-transporting ATPase subunit beta-m; ATP1b4 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of ATP1b4. No significant cross-reactivity or interference between ATP1b4 and analogues was observed.
Sequence Length
352
Samples
Tissue homogenates or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
78.125-5000pg/mL
Sensitivity
<27.8pg/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATP1b4 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATP1b4 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for ATP1b4 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of ATP1b4 in mouse tissue homogenates or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATP1b4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ATP1b4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATP1b4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ATP1b4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATP1b4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ATP1b4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATP1b4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ATP1b4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40,942 Da
NCBI Official Full Name
protein ATP1B4 isoform 2
NCBI Official Synonym Full Names
ATPase, (Na+)/K+ transporting, beta 4 polypeptide
NCBI Official Symbol
Atp1b4
NCBI Official Synonym Symbols
1110020B02Rik
NCBI Protein Information
protein ATP1B4
UniProt Protein Name
Protein ATP1B4
UniProt Gene Name
Atp1b4
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Product Notes
The Mouse ATP1b4 atp1b4 (Catalog #AAA68511) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA68511 ELISA Kit recognizes Mouse ATP1b4. It is sometimes possible for the material contained within the vial of "ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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