IF (Immunofluorescence)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant ATP5B protein using ATP5B antibody)
Mouse ATP5B Monoclonal Antibody | anti-ATP5B antibody
ATP5B antibody
Gene Names
ATP5B; ATPMB; ATPSB; HEL-S-271
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
ATP5B antibody was purified by affinity chromatography.
Synonyms
ATP5B, Antibody; ATP5B antibody; Monoclonal ATP5B; Anti-ATP5B; Atp Synthase H+ Transporting Mitochondrial F1 Complex Beta Polypeptide; ATPMB; ATPSB; MGC5231; ATP5B; ATPB 5; ATPB-5; anti-ATP5B antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
7F4
Purity/Purification
ATP5B antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (varies by lot)
Sequence Length
529
Applicable Applications for anti-ATP5B antibody
WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
Biological Significance
ATP5B is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel.
Cross-Reactivity
Human, Dog, Monkey
Immunogen
ATP5B antibody was raised in mouse using a full length recombinant protein of human ATP5B (NP_001677) produced in HEK293T cells, as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant ATP5B protein using ATP5B antibody)
IF (Immunofluorescence)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant ATP5B protein using ATP5B antibody)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of ATP5B protein in paraffin embedded Carcinoma of Human liver tissue using ATP5B antibody)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of ATP5B protein in paraffin embedded Carcinoma of Human liver tissue using ATP5B antibody)
WB (Western Blot)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ATP5B protein (Right) or empty vector (Left) detected with ATP5B antibody)
WB (Western Blot)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ATP5B protein (Right) or empty vector (Left) detected with ATP5B antibody)
Related Product Information for anti-ATP5B antibody
Mouse monoclonal ATP5B antibody
Product Categories/Family for anti-ATP5B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
56,560 Da
NCBI Official Full Name
ATP synthase subunit beta, mitochondrial
NCBI Official Synonym Full Names
ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide
NCBI Official Symbol
ATP5B
NCBI Official Synonym Symbols
ATPMB; ATPSB; HEL-S-271
NCBI Protein Information
ATP synthase subunit beta, mitochondrial
UniProt Protein Name
ATP synthase subunit beta, mitochondrial
UniProt Gene Name
ATP5B
UniProt Synonym Gene Names
ATPMB; ATPSB
UniProt Entry Name
ATPB_HUMAN
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Product Notes
The ATP5B atp5b (Catalog #AAA106510) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's ATP5B can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the ATP5B atp5b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATP5B, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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