Search results for "Proteases"

EPX, Polyclonal Antibody (Cat# AAA23997)

Full Name

EPX antibody

Gene Names

EPX; EPO; EPP; EPX-PEN

Purity

Affinity purified

Pricing

NNMT, Recombinant Protein (Cat# AAA11766)

Full Name

NNMT, 1-264aa, Human, His tag, E Coli

Applications

SDS-PAGE

Purity

> 95% by SDS-PAGE

Pricing

Thymidylate synthase, Recombinant Protein (Cat# AAA11790)

Full Name

Thymidylate synthase, 1-313aa, Human, His tag, E Coli

Gene Names

TYMS; TS; TMS; HST422

Applications

SDS-PAGE

Purity

> 95% by SDS-PAGE

Pricing

KLK4, Active Protein (Cat# AAA27762)

Full Name

KLK4 Protein, Human, Recombinant (His Tag)

Gene Names

KLK4; ARM1; EMSP; PSTS; AI2A1; EMSP1; KLK-L1; PRSS17; MGC116827; MGC116828

Purity

>94% as determined by SDS-PAGE

Pricing

GPI, Recombinant Protein (Cat# AAA11768)

Full Name

GPI, 1- 558aa, Human, His tag, E Coli

Gene Names

GPI; AMF; NLK; PGI; PHI; GNPI; SA36; SA-36

Applications

SDS-PAGE

Purity

> 95% by SDS-PAGE

Pricing

DPP4, Active Protein (Cat# AAA11760)

Full Name

DPP4 (CD26), 39-766aa, Human,His tag, Baculovirus (Bioactivity Validated)

Gene Names

DPP4; CD26; ADABP; ADCP2; DPPIV; TP103

Applications

SDS-PAGE

Purity

> 95% by SDS-PAGE

Pricing

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IF (Immunofluorescence) (ICC/IF analysis of FABP7 in PC3 cells. The cell was stained with AAA11731 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)

FABP7, Monoclonal Antibody (Cat# AAA11731)

Full Name

FABP7 antibody

Gene Names

FABP7; MRG; BLBP; FABPB; B-FABP

Reactivity

Human, Mouse

Applications

EIA, WB, ICC, IF

Purity

By protein-G affinity chromatography

Pricing

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IHC (Immunohistchemistry) (Immunochemical staining CD21 in cynomolgus lymph node with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

CD21, Polyclonal Antibody (Cat# AAA27739)

Full Name

Anti-CD21 Antibody, Rabbit Polyclonal

Gene Names

CR2; CR; C3DR; CD21; CVID7; SLEB9

Reactivity

Human, Cynomolgus

Applications

WB, EIA, IHC-P, IP

Purity

Protein A & Antigen Affinity

Pricing

alpha 1 Antitrypsin, Polyclonal Antibody (Cat# AAA14320)

Full Name

alpha 1 Antitrypsin antibody

Gene Names

SERPINA1; PI; A1A; AAT; PI1; A1AT; PRO2275; alpha1AT

Reactivity

Human

Applications

IHC

Pricing

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SDS-PAGE (SDS-PAGE Analysis Purified Calpastatin Mouse Monoclonal Antibody (CAST/1550). Confirmation of Integrity and Purity of Antibody.)

Calpastatin, Monoclonal Antibody (Cat# AAA23940)

Full Name

Calpastatin

Gene Names

CAST; BS-17; PLACK

Reactivity

Human

Applications

EIA, IHC

Purity

Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Pricing

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IHC (Immunohistochemistry) (Figure 7. IHC analysis of GALNS using anti-GALNS antibody (AAA19164).GALNS was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GALNS Antibody (AAA19164) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

GALNS, Antibody (Cat# AAA19164)

Full Name

Anti-GALNS Picoband antibody

Gene Names

GALNS; GAS; MPS4A; GalN6S; GALNAC6S

Reactivity

Reacts with: Human, Mouse, Rat

Applications

WB, EIA

Purity

Immunogen affinity purified

Pricing

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WB (Western Blot) (Sample: Recombinant PRF1, Human;Antibody: Rabbit Anti-Human PRF1 Ab)

Activity (Perforin 1 (PRF1) is a pore forming cytolytic protein found in the granules of cytotoxic T lymphocytes (CTLs) and NK cells. Upon degranulation, perforin binds to the target cell's plasma membrane, and oligomerises in a Ca2+ dependent manner to form pores on the target cell. The pore formed allows for the passive diffusion of a family of pro-apoptotic proteases, known as the granzymes, into the target cell. Besides, Calreticulin (CRT) has been identified as an interactor of PRF1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PRF1 and recombinant human CRT. Briefly, PRF1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CRT-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-PRF1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of PRF1 and CRT was shown in Figure 1, and this effect was in a dose dependent manner.The activity of recombinant PRF1 was measured by lysis of erythrocytes using a hemolysis assay. A general procedure is as fllows: two-fold dilute the recombinant human PRF1 with 0.9% NaCl, add 50uL a serial dilution of PRF1, 10uL 0.1M CaCl2 to each well, then add 50uL 0.25% rabbit erythrocyte (RaE) to each well and mixed gently. Add 10uL 0.9% NaCl to reaplace CaCl2 in control wells. The plate is incubated for 20 hours at 37 degree C, 5% CO2. The results are shown in Figure 2. It was obvious that the minimal effective concentration of PRF1 is 12.5ug/mL.)

Perforin 1 (PRF1), Active Protein (Cat# AAA21142)

Full Name

Active Perforin 1 (PRF1)

Gene Names

PRF1; P1; PFP; HPLH2

Reactivity

Homo sapiens (Human)

Applications

Cell culture; Activity Assays.

Purity

>80%

Pricing

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12 results for "Proteases" - showing 1-12

EPX, Polyclonal Antibody (Cat# AAA23997)

NNMT, Recombinant Protein (Cat# AAA11766)

Thymidylate synthase, Recombinant Protein (Cat# AAA11790)

KLK4, Active Protein (Cat# AAA27762)

GPI, Recombinant Protein (Cat# AAA11768)

DPP4, Active Protein (Cat# AAA11760)

FABP7, Monoclonal Antibody (Cat# AAA11731)

CD21, Polyclonal Antibody (Cat# AAA27739)

alpha 1 Antitrypsin, Polyclonal Antibody (Cat# AAA14320)

Calpastatin, Monoclonal Antibody (Cat# AAA23940)

GALNS, Antibody (Cat# AAA19164)

Perforin 1 (PRF1), Active Protein (Cat# AAA21142)

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Filters

Clonality

Type

Reactivity

Gene Name

Isotype

Host

Application

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12 results for "Proteases" - showing 1-12

EPX, Polyclonal Antibody (Cat# AAA23997)

NNMT, Recombinant Protein (Cat# AAA11766)

Thymidylate synthase, Recombinant Protein (Cat# AAA11790)

KLK4, Active Protein (Cat# AAA27762)

GPI, Recombinant Protein (Cat# AAA11768)

DPP4, Active Protein (Cat# AAA11760)

FABP7, Monoclonal Antibody (Cat# AAA11731)

CD21, Polyclonal Antibody (Cat# AAA27739)

alpha 1 Antitrypsin, Polyclonal Antibody (Cat# AAA14320)

Calpastatin, Monoclonal Antibody (Cat# AAA23940)

GALNS, Antibody (Cat# AAA19164)

Perforin 1 (PRF1), Active Protein (Cat# AAA21142)

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