Mouse b2-Microglobulin Monoclonal Antibody | anti-HLA-G antibody
b2-Microglobulin (B2M)
Gene Names
HLA-G; MHC-G
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
Purity
Purified
Synonyms
b2-Microglobulin, Antibody; b2-Microglobulin (B2M); Anti -b2-Microglobulin (B2M); anti-HLA-G antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
1.B.749
Specificity
Recognizes human Beta 2-microglobulin (MW 12kD). Does not react with Beta 2-microglobulin from other species. Recognizes the HLA-Beta 2-microglobulin complex, free or associated with the membrane. The exact epitope for the antibody has not been mapped.
Purity/Purification
Purified
Form/Format
Supplied as a lyophilized powder from PBS, 1mg/ml BSA. Reconstitute with 1ml sterile ddH2O.
Concentration
~0.2mg/ml (after reconstitution) (varies by lot)
Applicable Applications for anti-HLA-G antibody
Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes
Immunofluorescence/Flow Cytometry: 10ul/5x10e5 cells/test.
Immunohistochemistry: frozen sections of cell smears): 1:25-1:50
Optimal dilutions to be determined by the researcher.
Immunohistochemistry: frozen sections of cell smears): 1:25-1:50
Optimal dilutions to be determined by the researcher.
Immunogen
Purified human Beta 2-microglobulin
Hybridoma
NS1/Ag4.1 myeloma cells with spleen cells of Balb/c mice.
Grade
Purified
Preparation and Storage
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-HLA-G antibody
b2-microglobulin non-covalently associates with the 44kD a-chain to form the HLA Class I antigen complex. Human b2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.
Product Categories/Family for anti-HLA-G antibody
References
Activation of complement by monoclonal antibodies that targetcell-associated 2-microglobulin: Implications for cancerimmunotherapy. Michael J. Pokrass, Monica F. Liu, Margaret A. Lindorfer, Ronald P. Taylor (2013)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
b2 microglobulin
NCBI Official Synonym Full Names
major histocompatibility complex, class I, G
NCBI Official Symbol
HLA-G
NCBI Official Synonym Symbols
MHC-G
NCBI Protein Information
HLA class I histocompatibility antigen, alpha chain G; HLA G antigen; b2 microglobulin; OTTHUMP00000029218; OTTHUMP00000029220; OTTHUMP00000033527; OTTHUMP00000159507; HLA class I molecule; MHC class I antigen G; HLA-G histocompatibility antigen, class I, G
UniProt Protein Name
HLA-G protein
UniProt Gene Name
HLA-G
UniProt Entry Name
O02923_HUMAN
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Product Notes
The HLA-G hla-g (Catalog #AAA14654) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's b2-Microglobulin can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF). Immunofluorescence/Flow Cytometry: 10ul/5x10e5 cells/test. Immunohistochemistry: frozen sections of cell smears): 1:25-1:50 Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the HLA-G hla-g for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "b2-Microglobulin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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