ICC (Immunocytochemistry)
(Immunocytochemical labeling of B7-H3 in aldehyde fixed human MCF-7 breast ductal carcinoma cells. The cells were labeled with mouse monoclonal anti-B7-H3/CD276 (BM0451). The antibody was detected using goat anti-mouse DyLight 594.)
Mouse anti-Human B7-H3/CD276 Monoclonal Antibody | anti-B7-H3/CD276 antibody
B7-H3/CD276 (Extracellular region) Antibody
Gene Names
CD276; B7H3; B7-H3; B7RP-2; 4Ig-B7-H3
Reactivity
Human
Applications
Immunocytochemistry, Immunoprecipitation, ELISA, Western Blot
Purity
Protein G chromatography
Synonyms
B7-H3/CD276, Antibody; B7-H3/CD276 (Extracellular region) Antibody; anti-B7-H3/CD276 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
M045
Specificity
Clone M045 mouse monoclonal antibody detects a 100-120kDa* protein on SDS-PAGE "Native" or denatured immunoblots of human LNCaP, MCF-7, NCI-H28, A431, and MDA-MB-231 carcinomas. The antibody detects B7-H3 in membranes and cytoplasm in MCF7 cell after immunocytochemical labeling. The antibody works for western blot, immunoprecipitation, immunocytochemistry, and ELISA capture.
Purity/Purification
Protein G chromatography
Form/Format
Mouse monoclonal antibody purified with protein G chromatography is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-B7-H3/CD276 antibody
ICC (Immunocytochemistry), IP (Immunoprecipitation), ELISA, WB (Western Blot)
Immunogen
Clone (M045) was generated from a recombinant protein that included the extracellular region of human B7-H3 protein.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
ICC (Immunocytochemistry)
(Immunocytochemical labeling of B7-H3 in aldehyde fixed human MCF-7 breast ductal carcinoma cells. The cells were labeled with mouse monoclonal anti-B7-H3/CD276 (BM0451). The antibody was detected using goat anti-mouse DyLight 594.)
ICC (Immunocytochemistry)
(Immunocytochemical labeling of B7-H3 in aldehyde fixed human MCF-7 breast ductal carcinoma cells. The cells were labeled with mouse monoclonal anti-B7-H3/CD276 (BM0451). The antibody was detected using goat anti-mouse DyLight 594.)
WB (Western Blot)
(Western blot of human LNCaP prostate adenocarcinoma (lane 1), MCF-7 breast ductal carcinoma (lane 2), NCI-H28 pleural mesothelioma (lane 3), A431 epidermoid carcinoma (lane 4), MDAMB-231 breast carcinoma (lane 5) and Jurkat T-cell line (lane 6). The blot was probed with mouse monoclonal anti-B7-H3/CD276 (BM0451) at 1:500.)
WB (Western Blot)
(Western blot of human LNCaP prostate adenocarcinoma (lane 1), MCF-7 breast ductal carcinoma (lane 2), NCI-H28 pleural mesothelioma (lane 3), A431 epidermoid carcinoma (lane 4), MDAMB-231 breast carcinoma (lane 5) and Jurkat T-cell line (lane 6). The blot was probed with mouse monoclonal anti-B7-H3/CD276 (BM0451) at 1:500.)
Related Product Information for anti-B7-H3/CD276 antibody
B7 homolog 3 (B7-H3, CD276) is a member of the B7 family of cell surface ligands that regulate T cell activation and immune responses. B7-H3 is a membrane protein with an extracellular region that includes two Ig-like V-type domains and two IgG-like C2-type domains, and a short intracellular domain. B7-H3 is a regulatory molecule for immune reactions, such as T cell proliferation and IFN-gamma production. In colon cancers, B7-H3 inhibits T-cell cytotoxicity, and blocking B7-H3 function enhances T-cell cytotoxicity toward cancer cells. B7-H3 is expressed by antigen presenting cells, activated T cells, and a few normal tissues, including placenta and prostate. In cancer, B7-H3 is expressed in various tumor types including prostate, breast, colon, lung, and gastric cancers. The B7-H3 expression level correlates with tumor growth, invasion, metastasis, malignant stage, and recurrence rate. The inhibition or blockade of B7-H3 function could be an important immunotherapeutic approach for several types of cancer.
References
Chapoval AI, et al. (2001) Nat Immunol. 2(3):269.
Yang S. et al. (2020) Int J Biol Sci. 16(11):1767.
Yang S. et al. (2020) Int J Biol Sci. 16(11):1767.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
57,235 Da
NCBI Official Full Name
B7-H3 protein
NCBI Official Synonym Full Names
CD276 molecule
NCBI Official Symbol
CD276
NCBI Official Synonym Symbols
B7H3; B7-H3; B7RP-2; 4Ig-B7-H3
NCBI Protein Information
CD276 antigen; B7 homolog 3; costimulatory molecule
UniProt Protein Name
CD276 antigen
UniProt Gene Name
CD276
UniProt Synonym Gene Names
B7H3; B7-H3
UniProt Entry Name
CD276_HUMAN
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Product Notes
The B7-H3/CD276 cd276 (Catalog #AAA71571) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The B7-H3/CD276 (Extracellular region) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's B7-H3/CD276 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IP (Immunoprecipitation), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the B7-H3/CD276 cd276 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "B7-H3/CD276, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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