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WB (Western Blot)
(Detection of human B7-H4 by western blot. Samples: Whole cell lysate (50 ug) from OVCAR-4, Hep-G2, MCF-7, HeLa, HEK293T, Jurkat, MOLT-4, HH, and C5/MJ cells prepared using NETN lysis buffer. Antibody: Rabbit anti-B7-H4 recombinant monoclonal antibody (AAA23811 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds.)
B7-H4 recombinant antibody
Rabbit anti-B7-H4 Recombinant Monoclonal Antibody [BLR043F]
Gene Names
VTCN1; B7X; B7H4; B7S1; B7-H4; B7h.5; VCTN1; PRO1291
Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Purified
Synonyms
B7-H4; Monoclonal Recombinant Antibody; Rabbit anti-B7-H4 Recombinant Monoclonal Antibody [BLR043F]; PRO1291; B7S1; V-set domain-containing T-cell activation inhibitor 1; VCTN1; T-cell costimulatory molecule B7x; T cell costimulatory molecule B7x; Protein B7S1; B7 family member; H4; B7H4; B7h.5; B7 superfamily member 1; B7 homolog 4; immune costimulatory protein B7-H4; B7X; V-set domain containing T cell activation inhibitor 1; B7-H4 recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR043F
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
50ug/ml (varies by lot)
Applicable Applications for B7-H4 recombinant antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry-Immunofluorescence (IHC-IF), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000
IP: 20 ul/mg lysate
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
FC/FACS: Fixed and permeabilized cells were stained with 4 ul per 1 x 10^6 cells.
IP: 20 ul/mg lysate
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
FC/FACS: Fixed and permeabilized cells were stained with 4 ul per 1 x 10^6 cells.
Immunogen
Residues 30-282 (ECD)
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human B7-H4 by western blot. Samples: Whole cell lysate (50 ug) from OVCAR-4, Hep-G2, MCF-7, HeLa, HEK293T, Jurkat, MOLT-4, HH, and C5/MJ cells prepared using NETN lysis buffer. Antibody: Rabbit anti-B7-H4 recombinant monoclonal antibody (AAA23811 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds.)
WB (Western Blot)
(Detection of human B7-H4 by western blot. Samples: Whole cell lysate (50 ug) from OVCAR-4, Hep-G2, MCF-7, HeLa, HEK293T, Jurkat, MOLT-4, HH, and C5/MJ cells prepared using NETN lysis buffer. Antibody: Rabbit anti-B7-H4 recombinant monoclonal antibody (AAA23811 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human B7-H4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from OVCAR-4 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-B7-H4 recombinant monoclonal antibody (AAA23811 lot 1) used for IP at 20 ul per reaction. B7-H4 was also immunoprecipitated by rabbit anti-B7-H4 recombinant monoclonal antibody [209-2B5]. For blotting immunoprecipitated B7-H4, AAA23811 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human B7-H4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from OVCAR-4 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-B7-H4 recombinant monoclonal antibody (AAA23811 lot 1) used for IP at 20 ul per reaction. B7-H4 was also immunoprecipitated by rabbit anti-B7-H4 recombinant monoclonal antibody [209-2B5]. For blotting immunoprecipitated B7-H4, AAA23811 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IHC (Immunohistochemistry)
(Detection of human B7-H4 (red) by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Rabbit anti-B7-H4 recombinant monoclonal antibody (AAA23811 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI (blue).)
IHC (Immunohistochemistry)
(Detection of human B7-H4 (red) by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Rabbit anti-B7-H4 recombinant monoclonal antibody (AAA23811 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI (blue).)
IHC (Immunohistochemistry)
(Detection of human B7-H4 by immunohistochemistry. Sample: FFPE section of ovarian carcinoma. Antibody: Rabbit anti-B7-H4 recombinant monoclonal antibody (AAA23811 lot 1) used at 1:100. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of human B7-H4 by immunohistochemistry. Sample: FFPE section of ovarian carcinoma. Antibody: Rabbit anti-B7-H4 recombinant monoclonal antibody (AAA23811 lot 1) used at 1:100. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human B7-H4 by immunocytochemistry. Sample: FFPE section of OVCAR-3 cells. Antibody: Rabbit anti-B7-H4 recombinant monoclonal antibody (AAA23811 lot 1) used at 1:100. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human B7-H4 by immunocytochemistry. Sample: FFPE section of OVCAR-3 cells. Antibody: Rabbit anti-B7-H4 recombinant monoclonal antibody (AAA23811 lot 1) used at 1:100. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
FCM (Flow Cytometry)
(Detection of human B7-H4 (shaded) in human peripheral blood mononuclear cells by flow cytometry. Antibody: Rabbit anti-B7-H4 recombinant monoclonal (AAA23811 lot 1) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry)
(Detection of human B7-H4 (shaded) in human peripheral blood mononuclear cells by flow cytometry. Antibody: Rabbit anti-B7-H4 recombinant monoclonal (AAA23811 lot 1) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
Related Product Information for B7-H4 recombinant antibody
B7-H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, B7-H4 plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P10914].
Product Categories/Family for B7-H4 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
V-set domain-containing T-cell activation inhibitor 1 isoform 1
NCBI Official Synonym Full Names
V-set domain containing T cell activation inhibitor 1
NCBI Official Symbol
VTCN1
NCBI Official Synonym Symbols
B7X; B7H4; B7S1; B7-H4; B7h.5; VCTN1; PRO1291
NCBI Protein Information
V-set domain-containing T-cell activation inhibitor 1; VTCN1; B7 homolog 4; B7 family member, H4; B7 superfamily member 1; T cell costimulatory molecule B7x; T-cell costimulatory molecule B7x; immune costimulatory protein B7-H4
UniProt Protein Name
V-set domain-containing T-cell activation inhibitor 1
UniProt Gene Name
VTCN1
UniProt Synonym Gene Names
B7H4
UniProt Entry Name
VTCN1_HUMAN
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Product Notes
The B7-H4 vtcn1 (Catalog #AAA23811) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-B7-H4 Recombinant Monoclonal Antibody [BLR043F] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's B7-H4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry-Immunofluorescence (IHC-IF), Flow Cytometry (FC/FACS). WB: 1:1000 IP: 20 ul/mg lysate IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. FC/FACS: Fixed and permeabilized cells were stained with 4 ul per 1 x 10^6 cells. Researchers should empirically determine the suitability of the B7-H4 vtcn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "B7-H4, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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