FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of K562 cells using anti-BAG2 antibody (AAA126942).Overlay histogram showing K562 cells stained with AAA126942 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-BAG2 Antibody (AAA126942, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Mouse anti-Human, Mouse BAG2 Monoclonal Antibody | anti-BAG2 antibody
Anti-BAG2 Antibody Picoband (monoclonal, 8F11G2)
Gene Names
BAG2; BAG-2; dJ417I1.2
Reactivity
Human, Mouse
Applications
Flow Cytometry, Functional Assay, Western Blot
Purity
Immunogen affinity purified.
Synonyms
BAG2, Antibody; Anti-BAG2 Antibody Picoband (monoclonal, 8F11G2); anti-BAG2 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
8F11G2
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. (varies by lot)
Applicable Applications for anti-BAG2 antibody
FCM/FACS (Flow Cytometry), WB (Western Blot)
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Immunogen
E Coli-derived human BAG2 recombinant protein (Position: M1-N211). Human BAG2 shares 93.4% amino acid (aa) sequence identity with mouse BAG2.
Preparation and Storage
Store at -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months. Avoid repeated freezing and thawing.
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of K562 cells using anti-BAG2 antibody (AAA126942).Overlay histogram showing K562 cells stained with AAA126942 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-BAG2 Antibody (AAA126942, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of K562 cells using anti-BAG2 antibody (AAA126942).Overlay histogram showing K562 cells stained with AAA126942 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-BAG2 Antibody (AAA126942, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
WB (Western Blot)
(Figure 1. Western blot analysis of BAG2 using anti-BAG2 antibody (AAA126942).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Jurkat whole cell lysates,Lane 2: human Hela whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: human K562 whole cell lysates,Lane 5: mouse liver tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-BAG2 antigen affinity purified monoclonal antibody (#AAA126942) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BAG2 at approximately 24 kDa. The expected band size for BAG2 is at 24 kDa.)
WB (Western Blot)
(Figure 1. Western blot analysis of BAG2 using anti-BAG2 antibody (AAA126942).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Jurkat whole cell lysates,Lane 2: human Hela whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: human K562 whole cell lysates,Lane 5: mouse liver tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-BAG2 antigen affinity purified monoclonal antibody (#AAA126942) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BAG2 at approximately 24 kDa. The expected band size for BAG2 is at 24 kDa.)
Related Product Information for anti-BAG2 antibody
BAG family molecular chaperone regulator 2 is a protein that in humans is encoded by the BAG2 gene. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. The functional antagonisms displayed between BAG family proteins and Hip suggest that a proper balance of these 2 types of protein is required for achieving optimal cycles of substrate binding and release required for inducting conformational changes in proteins, with Hip promoting peptide substrate binding by Hsc70/Hsp70 and BAG family proteins promoting dissociation.
Product Categories/Family for anti-BAG2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20,189 Da
NCBI Official Full Name
BAG family molecular chaperone regulator 2
NCBI Official Synonym Full Names
BCL2-associated athanogene 2
NCBI Official Symbol
BAG2
NCBI Official Synonym Symbols
BAG-2; dJ417I1.2
NCBI Protein Information
BAG family molecular chaperone regulator 2; BAG-family molecular chaperone regulator-2; bcl-2-associated athanogene 2; dJ417I1.2 (BAG-family molecular chaperone regulator 2)
UniProt Protein Name
BAG family molecular chaperone regulator 2
UniProt Gene Name
BAG2
UniProt Synonym Gene Names
BAG-2
UniProt Entry Name
BAG2_HUMAN
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Product Notes
The BAG2 bag2 (Catalog #AAA126942) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-BAG2 Antibody Picoband (monoclonal, 8F11G2) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's BAG2 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the BAG2 bag2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BAG2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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