IHC (Immunohiostchemistry)
(IHC image of CSB-RA002531A0HU diluted at 1:73.75 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
BAG3 recombinant antibody
BAG3 Antibody
Gene Names
BAG3; BIS; MFM6; BAG-3; CAIR-1
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Affinity-Chromatography
Synonyms
BAG3, Recombinant Antibody; BAG3 Antibody; BAG family molecular chaperone regulator 3; BAG-3; Bcl-2-associated athanogene 3; Bcl-2-binding protein Bis; Docking protein CAIR-1; BAG3; BIS; BAG3 recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Purity/Purification
Affinity-Chromatography
Form/Format
Liquid; In phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
575
Applicable Applications for BAG3 recombinant antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA
Immunogen Species
Human
Immunogen
A synthesized peptide derived from human BAG3
Conjugate
Non-Conjugated
Preparation and Storage
Upon receipt, store at-20 degree C or-80 degree C. Avoid repeated freeze.
IHC (Immunohiostchemistry)
(IHC image of CSB-RA002531A0HU diluted at 1:73.75 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohiostchemistry)
(IHC image of CSB-RA002531A0HU diluted at 1:73.75 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
WB (Western Blot)
(Western BlotPositive WB detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, U87 whole cell lysateAll lanes: BAG3 antibody at 0.73ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 62 KDaObserved band size: 80 KDa)
WB (Western Blot)
(Western BlotPositive WB detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, U87 whole cell lysateAll lanes: BAG3 antibody at 0.73ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 62 KDaObserved band size: 80 KDa)
Related Product Information for BAG3 recombinant antibody
Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release. Nucleotide release is mediated via its binding to the nucleotide-binding domain (NBD) of HSPA8/HSC70 where as the substrate release is mediated via its binding to the substrate-binding domain (SBD) of HSPA8/HSC70 (PubMed:9873016, PubMed:27474739). Has anti-apoptotic activity (PubMed:10597216). Plays a role in the HSF1 nucleocytoplasmic transport (PubMed:26159920).
Product Categories/Family for BAG3 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
61,595 Da
NCBI Official Full Name
BAG family molecular chaperone regulator 3
NCBI Official Synonym Full Names
BCL2 associated athanogene 3
NCBI Official Symbol
BAG3
NCBI Official Synonym Symbols
BIS; MFM6; BAG-3; CAIR-1
NCBI Protein Information
BAG family molecular chaperone regulator 3
UniProt Protein Name
BAG family molecular chaperone regulator 3
UniProt Gene Name
BAG3
UniProt Synonym Gene Names
BIS; BAG-3
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Product Notes
The BAG3 bag3 (Catalog #AAA235525) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BAG3 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BAG3 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the BAG3 bag3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BAG3, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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