Mouse Basigin / Emmprin / CD147 Monoclonal Antibody | anti-BSG antibody

Mouse Monoclonal [clone MEM-M6/2] (IgG2b) to Human Basigin / Emmprin / CD147

Cat. Num. AAA51783

• Gene Names: BSG; M6; OK; 5F7; TCSF; CD147; EMMPRIN
• Reactivity: Human, Porcine
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry
• Purity: Purified by protein-A affinity chromatography. Greater than 95% by SDS-PAGE.
• Synonyms: Basigin / Emmprin / CD147, Antibody; Mouse Monoclonal [clone MEM-M6/2] (IgG2b) to Human Basigin / Emmprin / CD147; Anti-Basigin / Emmprin / CD147 Antibody (clone MEM-M6/2) IHC-plus; BSG; 5F7; Basigin; Basigin (Ok blood group); CD147 antigen; Collagenase stimulatory factor; EMMPRIN; OK; OK blood group antigen; TCSF; CD147; M6; Emmprin; Human Basigin; anti-BSG antibody

• Host: Mouse
• Reactivity: Human, Porcine
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: MEM-M6/2
• Specificity: The antibody MEM-M6/2 recognizes extracellular Ig domain D1 of CD147 (Neurothelin), a 50-60 kDa type I transmembrane glycoprotein primarily expressed on all leukocytes, red blood cells, platelets and endothelial cells; it is not expressed by resting lymphocytes. The antibody MEM-M6/1 is a high-affinity antibody capable of binding to unstimulated peripheral blood T cells.
• Purity/Purification: Purified by protein-A affinity chromatography. Greater than 95% by SDS-PAGE.
• Form/Format: PBS, pH 7.4, 15mM Sodium Azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 385

• Applicable Applications for anti-BSG antibody: FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry)
• Antigen Type: Recombinant protein
• Immunogen Species: Human
• Immunogen: Basigin / Emmprin / CD147 antibody was raised against a soluble recombinant form of CD147, CD147Rg, which consists of the cDNA coding for the entire extracellular region of CD147 fused to the DNA coding for the hinge region, CH2 and CH3 domain of human IgG1.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at 2°C to 8°C. Do not freeze.

IHC (Immunohistochemistry)

(Anti-CD147 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Related Product Information for anti-BSG antibody

CD147 (BSG) is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. The encoded protein is also a member of the immunoglobulin superfamily. Multiple transcript variants encoding different isoforms have been found for this gene.

NCBI and Uniprot Product Information

• NCBI GI #: 38372919
• NCBI GeneID: 682
• NCBI Accession #: NP_001719.2
• NCBI GenBank Nucleotide #: NM_001728.3
• UniProt Accession #: P35613; Q7Z796; Q8IZL7; A6NJW1; D3YLG5
• NCBI Official Full Name: basigin isoform 1
• NCBI Official Synonym Full Names: basigin (Ok blood group)
• NCBI Official Symbol: BSG
• NCBI Official Synonym Symbols: M6; OK; 5F7; TCSF; CD147; EMMPRIN
• NCBI Protein Information: basigin; CD147 antigen; OK blood group antigen; collagenase stimulatory factor; leukocyte activation antigen M6; extracellular matrix metalloproteinase inducer; tumor cell-derived collagenase stimulatory factor
• UniProt Protein Name: Basigin
• UniProt Gene Name: BSG
• UniProt Synonym Gene Names: EMMPRIN; TCSF
• UniProt Entry Name: BASI_HUMAN