WB (Western Blot)
(Western blot analysis of lysates from 1) Hela Cell Lysate. 2) C2C12 Cell Lysate. 3) PC12 Cell Lysate cells. (Green) primary antibody was diluted at 1:1000. 4 degree over night.Dylight 800 secondary antibody(Assay Biotech:SA0438)was diluted at 1:10000. 37 degree 1hour. (Red) Actin ? Polyclonal Antibody (Assay Biotech:C40022) antibody was diluted at 1:5000 as loading control. 4 degree over night.Dylight 680 secondary antibody(Assay Biotech:SA0437)was diluted at 1:10000. 37 degree 1hour.)
Mouse anti-Human Bax Monoclonal Antibody | anti-Bax antibody
Bax (PTR1159) Mouse Monoclonal Antibody
Gene Names
BAX; BCL2L4
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, Immunocytochemistry, Immunofluorescence
Purity
Protein G
Synonyms
Bax, Antibody; Bax (PTR1159) Mouse Monoclonal Antibody; anti-Bax antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1, kappa
Clone Number
PTR1159
Specificity
This antibody detects endogenous levels of bax.
Purity/Purification
Protein G
Form/Format
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-Bax antibody
IHC (Immunohistochemistry), WB (Western Blot), ICC (Immunocytochemistry), IF (Immunofluorescence)
Immunogen
Synthetic Peptide of Bax at AA range of 30-110
Modification
Unmodified/Total
Preparation and Storage
Store at -20 degree C/1 year
WB (Western Blot)
(Western blot analysis of lysates from 1) Hela Cell Lysate. 2) C2C12 Cell Lysate. 3) PC12 Cell Lysate cells. (Green) primary antibody was diluted at 1:1000. 4 degree over night.Dylight 800 secondary antibody(Assay Biotech:SA0438)was diluted at 1:10000. 37 degree 1hour. (Red) Actin ? Polyclonal Antibody (Assay Biotech:C40022) antibody was diluted at 1:5000 as loading control. 4 degree over night.Dylight 680 secondary antibody(Assay Biotech:SA0437)was diluted at 1:10000. 37 degree 1hour.)
WB (Western Blot)
(Western blot analysis of lysates from 1) Hela Cell Lysate. 2) C2C12 Cell Lysate. 3) PC12 Cell Lysate cells. (Green) primary antibody was diluted at 1:1000. 4 degree over night.Dylight 800 secondary antibody(Assay Biotech:SA0438)was diluted at 1:10000. 37 degree 1hour. (Red) Actin ? Polyclonal Antibody (Assay Biotech:C40022) antibody was diluted at 1:5000 as loading control. 4 degree over night.Dylight 680 secondary antibody(Assay Biotech:SA0437)was diluted at 1:10000. 37 degree 1hour.)
WB (Western Blot)
(Western Blot analysis of chicken cell lysis using Antibody diluted at 1:1000)
WB (Western Blot)
(Western Blot analysis of chicken cell lysis using Antibody diluted at 1:1000)
WB (Western Blot)
(Western blot analysis of 1) Hela Cell Lysate. 2) C2C12 Cell Lysate. 3) PC12 Cell Lysate using Bax Mouse mAb diluted at 1:1000.)
WB (Western Blot)
(Western blot analysis of 1) Hela Cell Lysate. 2) C2C12 Cell Lysate. 3) PC12 Cell Lysate using Bax Mouse mAb diluted at 1:1000.)
Related Product Information for anti-Bax antibody
The protein encoded by BAX (BCL2 associated X, apoptosis regulator) belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for BAX.
Product Categories/Family for anti-Bax antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
21,184 Da
NCBI Official Full Name
apoptosis regulator BAX isoform beta
NCBI Official Synonym Full Names
BCL2-associated X protein
NCBI Official Symbol
BAX
NCBI Official Synonym Symbols
BCL2L4
NCBI Protein Information
apoptosis regulator BAX; bcl2-L-4; bcl-2-like protein 4; BCL2-associated X protein omega; BCL2-associated X protein transcript variant delta2
UniProt Protein Name
Apoptosis regulator BAX
UniProt Gene Name
BAX
UniProt Synonym Gene Names
BCL2L4; Bcl2-L-4
UniProt Entry Name
BAX_HUMAN
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Product Notes
The Bax bax (Catalog #AAA320512) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Bax (PTR1159) Mouse Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Bax can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ICC (Immunocytochemistry), IF (Immunofluorescence). Researchers should empirically determine the suitability of the Bax bax for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bax, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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