Application Data
Mouse anti-Human BCA-225 Monoclonal Antibody | anti-BCA-225 antibody
BCA-225
Reactivity
Human
Applications
Immunohistochemistry
Synonyms
BCA-225, Antibody; BCA-225; anti-BCA-225 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1/K
Clone Number
Cu-18
Form/Format
BCA-225 is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Applicable Applications for anti-BCA-225 antibody
IHC (Immunohistochemistry)
Control
Breast, Lung, Uterus, Cervical Carcinoma, Breast Carcinoma
Localization
Cytoplasmic
Reactivity Note
Paraffin, Frozen
Immunogen
BCA 225 protein secreted by the T47D (clone 11) human breast carcinoma cell line.
Precautions
1. This product contains <0.1% sodium azide (NaN3) as a preservative. Ensure proper handling procedures are used with this reagent.
2. Always wear personal protective equipment such as laboratory coat, goggles and gloves when handling reagents.
3. Dispose of unused solution with copious amount of water.
4. Do not ingest reagent. If reagent is ingested, seek medical advice immediately.
5. Avoid contact with eyes. If contact occurs, flush with large quantities of water.
6. Follow safety precautions of the heating device used for epitope retrieval (TintoRetriever Pressure Cooker or similar).
7. For additional safety information refer to Safety Data Sheet for this product.
8. For complete recommendations for handling biological specimens, please refer to the CDC document, “Guidelines for Safe Work Practices in Human and Animal Laboratories” (see References in this document).
2. Always wear personal protective equipment such as laboratory coat, goggles and gloves when handling reagents.
3. Dispose of unused solution with copious amount of water.
4. Do not ingest reagent. If reagent is ingested, seek medical advice immediately.
5. Avoid contact with eyes. If contact occurs, flush with large quantities of water.
6. Follow safety precautions of the heating device used for epitope retrieval (TintoRetriever Pressure Cooker or similar).
7. For additional safety information refer to Safety Data Sheet for this product.
8. For complete recommendations for handling biological specimens, please refer to the CDC document, “Guidelines for Safe Work Practices in Human and Animal Laboratories” (see References in this document).
Mounting Protocols
For detailed instructions using biodegradable permanent mounting media such as XyGreen PermaMounter or organic solvent based resin such as PermaMounter, please inquire.
Preparation and Storage
Store at 2-8°C. (Control Slides: Store at 20-25°C)
Application Data
Application Data
IHC (Immunohistochemistry)
(Immunohistochemistry of BCA-225 on a FFPE Breast Carcinoma Tissue)
IHC (Immunohistochemistry)
(Immunohistochemistry of BCA-225 on a FFPE Breast Carcinoma Tissue)
Related Product Information for anti-BCA-225 antibody
This antibody recognizes a human Breast Carcinoma-associated glycoprotein, BCA-225 (220-225 kDa). This protein diff ers in size and distribution from other Breast Carcinoma antigens. Unlike other carcinoma antibodies against Breast Carcinoma antigens, this antibody does not react with benign or malignant colonic tissues. Since this antigen is localized in malignancies of Breast Carcinomas and Carcinoma of the Uterine Cervix, it can be eff ectively used to identify metastatic Breast Carcinoma lesions. Strong intracytoplasmic staining is seen in primary and metastatic Breast Carcinoma tissue, as well as in Cervical Carcinomas. Apical staining is seen in normal kidney, lung, Fallopian tube, liver, skin (eccrine sweat glands) and uterus. Similar staining patterns are observed in lung, ovarian, and endometrial cancers. Carcino-mas of the colon, stomach, prostate, urinary bladder, liver, pancreas, thyroid, and parotid are negative, as are Sarcomas and Lymphoid Cancers.
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Product Notes
The BCA-225 (Catalog #AAA59170) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The BCA-225 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BCA-225 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the BCA-225 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BCA-225, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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