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WB (Western Blot)
(Detection of human Bcl11a by western blot. Samples: Whole cell lysate (50 ug) from Ramos, HEK293T, Jurkat, MOLT-4, and Raji cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Bcl11a recombinant monoclonal antibody (AAA213561 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
BCL11A recombinant antibody
Rabbit anti-Bcl11a Recombinant Monoclonal Antibody [BLR073G]
Gene Names
BCL11A; EVI9; CTIP1; ZNF856; HBFQTL5; BCL11A-L; BCL11A-S; BCL11a-M; BCL11A-XL
Reactivity
Human, Mouse
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
Bcl11a, Recombinant Antibody; Rabbit anti-Bcl11a Recombinant Monoclonal Antibody [BLR073G]; C2H2-type zinc finger protein; zinc finger protein 856; HBFQTL5; EVI-9; EVI9; ecotropic viral integration site 9 protein homolog; ecotropic viral integration site 9 homolog; DILOS; ZNF856; COUP-TF-interacting protein 1; B-cell CLL/lymphoma 11A (zinc finger protein) isoform 2; CTIP1; B-cell CLL/lymphoma 11A; B-cell lymphoma/leukemia 11A; BCL-11A; BCL11A B-cell CLL/lymphoma 11A (zinc finger protein) isoform 1; BCL11A-L; BCL11a-M; BCL11A-S; BCL11A-XL; B cell CLL/lymphoma 11A; BCL11A recombinant antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Monoclonal
Clone Number
BLR073G
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Concentration
1000ug/ml (varies by lot)
Applicable Applications for BCL11A recombinant antibody
FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human Bcl11a by western blot. Samples: Whole cell lysate (50 ug) from Ramos, HEK293T, Jurkat, MOLT-4, and Raji cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Bcl11a recombinant monoclonal antibody (AAA213561 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot)
(Detection of human Bcl11a by western blot. Samples: Whole cell lysate (50 ug) from Ramos, HEK293T, Jurkat, MOLT-4, and Raji cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Bcl11a recombinant monoclonal antibody (AAA213561 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot)
(Detection of human Bcl11a by western blot. Samples: Whole cell lysate (50 ug) from A20, BW5147.3, EL4, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Bcl11a recombinant monoclonal antibody (AAA213561 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot)
(Detection of human Bcl11a by western blot. Samples: Whole cell lysate (50 ug) from A20, BW5147.3, EL4, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Bcl11a recombinant monoclonal antibody (AAA213561 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody .)
IP (Immunoprecipitation)
(Detection of human Bcl11a by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Ramos cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-Bcl11a recombinant monoclonal antibody (AAA213561 lot 2) used for IP at 20 ul/mg lysate. Bcl11a was also immunoprecipitated by a previous lot of this antibody (AAA213561 lot 1) and a second antibody against a different epitope of Bcl11a . For blotting immunoprecipitated Bcl11a, AAA213561 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human Bcl11a by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Ramos cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-Bcl11a recombinant monoclonal antibody (AAA213561 lot 2) used for IP at 20 ul/mg lysate. Bcl11a was also immunoprecipitated by a previous lot of this antibody (AAA213561 lot 1) and a second antibody against a different epitope of Bcl11a . For blotting immunoprecipitated Bcl11a, AAA213561 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
FCM/FACS (Flow Cytometry)
(Detection of human Bcl11a (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-Bcl11a recombinant monoclonal (AAA213561 lot 1) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
FCM/FACS (Flow Cytometry)
(Detection of human Bcl11a (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-Bcl11a recombinant monoclonal (AAA213561 lot 1) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
Related Product Information for BCL11A recombinant antibody
Bcl11a is a transcription factor that functions in B cell hematopoiesis and the generation of B lineage progenitors. Bcl11a has been identified as an oncogene involved in lymphoma pathogenesis and may function as a modulator of ARP1 transcriptional repression.
Product Categories/Family for BCL11A recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
87,554 Da
NCBI Official Full Name
B-cell lymphoma/leukemia 11A isoform 3
NCBI Official Synonym Full Names
B-cell CLL/lymphoma 11A
NCBI Official Symbol
BCL11A
NCBI Official Synonym Symbols
EVI9; CTIP1; ZNF856; HBFQTL5; BCL11A-L; BCL11A-S; BCL11a-M; BCL11A-XL
NCBI Protein Information
B-cell lymphoma/leukemia 11A
UniProt Protein Name
B-cell lymphoma/leukemia 11A
UniProt Gene Name
BCL11A
UniProt Synonym Gene Names
CTIP1; EVI9; KIAA1809; ZNF856; BCL-11A; EVI-9
UniProt Entry Name
BC11A_HUMAN
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Product Notes
The BCL11A bcl11a (Catalog #AAA213561) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Bcl11a Recombinant Monoclonal Antibody [BLR073G] reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Bcl11a can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the BCL11A bcl11a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bcl11a, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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