Mouse anti-Human Beta-2 microglobulin Monoclonal Antibody | anti-B2M antibody

Anti-Beta-2 microglobulin Antibody, Mouse Monoclonal

Cat. Num. AAA255495

• Reactivity: Human
• Applications: Immunofluorescence, Immunocytochemistry, Flow Cytometry, Functional Assay, ELISA, Western Blot
• Purity: Protein A
• Synonyms: Beta-2 microglobulin, Antibody; Anti-Beta-2 microglobulin Antibody, Mouse Monoclonal; Beta-2 microglobulin/B2M Antibody, Mouse MAb; beta-2 microglobulin; Anti-Beta-2 microglobulin Antibody; anti-B2M antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 35
• Specificity: Human Beta-2 microglobulin/B2M
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-B2M antibody: IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
• Immunogen: Recombinant Human Beta-2 microglobulin/B2M Protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Beta-2 microglobulin/B2M (rh Beta-2 microglobulin/B2M). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(Flow cytometric analysis of Human B2M / Beta-2-microglobulin expression on human whole blood lymphocytes. Cells were stained with purified anti-Human B2M / Beta-2-microglobulin, then a PE-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.)

IF (Immunofluorescence)

(Immunofluorescence staining of B2M in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with mouse anti-human B2M monoclonal antibody (dilution ratio 1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor?488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to Cytoplasm.)

WB (Western Blot)

(Anti-B2M mouse monoclonal antibody at 1:500 dilutionLane A: A-431 Whole Cell LysateLane B: U-937 Whole Cell LysateLane C: Raji Whole Cell LysateLane D: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:13 kDaObserved band size:13 kDa)

Related Product Information for anti-B2M antibody

B2M, also known as beta2-Microglobulin or CDABP0092, is a component of MHC class I molecules found expression in all nucleated cells (excludes red blood cells). The major function of MHC class I moleculesis is to display fragments of proteins from within the cell to T-cells and cells containing foreign proteins will be attacked. B2M (beta2-Microglobulin) is a low molecular weight protein. It was demonstrated that B2M (beta2-Microglobulin) was localized in the membranes of nucleated cells and was found to be associated with HL-A antigens. B2M (beta2-Microglobulin) is present in free form in various body fluids and as a subunit of histocompatibility antigens on cell surfaces lateral to the alpha3 chain. Unlike alpha3, beta2 has no transmembrane region. Directly above beta2 lies the alpha1 chain, which itself is lateral to the alpha2. In the absence of B2M (beta2 microglobulin), very limited amounts of MHC class I (classical and non-classical) molecules can be detected on the surface. In the absence of MHC class I, CD8 T cells, a subset of T cells involved in the development of acquired immunity cannot develop. Low levels of B2M (beta2 microglobulin) can indicate non-progression of HIV.

References

Poulik MD, et al. (1979) Beta 2-Microglobulin: methods and clinical applications. CRC Ctit Rev Clin Lab Sci. 10(3): 225-45.Poulik MD, et al. (1975) Beta2-Microglobulins. Contemp Top Mol Immunol. 4: 157-204.Berggard I. (1976) Beta2-Microglobulins: isolation, properties, and distribution. Fed Proc. 35(5): 1167-70.

NCBI and Uniprot Product Information

• NCBI GI #: 4757826
• NCBI GeneID: 567
• NCBI Accession #: NP_004039.1
• NCBI GenBank Nucleotide #: NM_004048.2
• UniProt Accession #: P61769; P01884; Q540F8; Q6IAT8; Q9UCK0; Q9UD48; Q9UDF4
• NCBI Official Full Name: beta-2-microglobulin
• NCBI Official Synonym Full Names: beta-2-microglobulin
• NCBI Official Symbol: B2M
• NCBI Protein Information: beta-2-microglobulin; beta chain of MHC class I molecules; beta-2-microglobin
• UniProt Protein Name: Beta-2-microglobulin
• UniProt Gene Name: B2M
• UniProt Entry Name: B2MG_HUMAN