FCM/FACS (Flow Cytometry)
(Human HepG2 cell line was fixed with 2% paraformaldehyde (10 min), permeabilised with 0.1% BSA-Triton X-100,then stained with 20ug/ml rabbit Anti-human a1AGP Polyclonal Antibody ( #AAA162120, red histogram) or Isotype control antibody , followed by 1ug/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody .)
Rabbit anti-Human Beta-2-Microglobulin (b2M) Polyclonal Antibody | anti-b2M antibody
Polyclonal Antibody to Beta-2-Microglobulin (b2M)
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Beta-2-Microglobulin (b2M), Antibody; Polyclonal Antibody to Beta-2-Microglobulin (b2M); Beta-2-Microglobulin; BMG; B2MG; anti-b2M antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against b2M. It has been selected for its ability to recognize b2M in immunohistochemical staining and western blotting.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Liquid; PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
0.5mg/mL (varies by lot)
Sequence
Gln22~Met119
Applicable Applications for anti-b2M antibody
FCM/FACS (Flow Cytometry)
Immunogen
Recombinant Beta-2-Microglobulin (b2M)
Conjugation
Unconjugated
Research Area
Infection immunity, Kidney biomarker
Preparation and Storage
Store at 4 degree C for frequent use. Store at -20 degree C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
FCM/FACS (Flow Cytometry)
(Human HepG2 cell line was fixed with 2% paraformaldehyde (10 min), permeabilised with 0.1% BSA-Triton X-100,then stained with 20ug/ml rabbit Anti-human a1AGP Polyclonal Antibody ( #AAA162120, red histogram) or Isotype control antibody , followed by 1ug/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody .)
FCM/FACS (Flow Cytometry)
(Human HepG2 cell line was fixed with 2% paraformaldehyde (10 min), permeabilised with 0.1% BSA-Triton X-100,then stained with 20ug/ml rabbit Anti-human a1AGP Polyclonal Antibody ( #AAA162120, red histogram) or Isotype control antibody , followed by 1ug/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody .)
WB (Western Blot)
(Western Blot; Sample: Human PlasmaPrimary Ab: 0.1ug/ml Rabbit Anti-Human b2M AntibodySecond Ab: 0.2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot; Sample: Human PlasmaPrimary Ab: 0.1ug/ml Rabbit Anti-Human b2M AntibodySecond Ab: 0.2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Product Categories/Family for anti-b2M antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
119
NCBI Official Full Name
Beta-2-microglobulin
NCBI Official Synonym Full Names
beta-2-microglobulin
NCBI Official Symbol
B2M
NCBI Protein Information
beta-2-microglobulin; beta-2-microglobin; beta chain of MHC class I molecules
UniProt Protein Name
Beta-2-microglobulin
UniProt Gene Name
B2M
UniProt Entry Name
B2MG_HUMAN
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Product Notes
The b2M b2m (Catalog #AAA162120) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Beta-2-Microglobulin (b2M) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Beta-2-Microglobulin (b2M) can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the b2M b2m for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Gln22~Met1 19. It is sometimes possible for the material contained within the vial of "Beta-2-Microglobulin (b2M), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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