WB (Western Blot)
(Western Blot using anti-Beta-2 microglobulin antibody BBM.1 HeLa cell lysate (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of BBM.1 (AAA72020) at 0.03 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Beta-2 microglobulin is 13.7 kDa. AAA72020 successfully detected human Beta-2 microglobulin in both HeLa cell extract and human duodenum samples.)
Beta-2 Microglobulin Recombinant Antibody | B2M recombinant antibody
Anti-Beta-2 Microglobulin [BBM.1]
Gene Names
B2M; IMD43
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, Immunofluorescence
Purity
Protein A Affinity Purified
Synonyms
Beta-2 Microglobulin, Recombinant Antibody; Anti-Beta-2 Microglobulin [BBM.1]; Beta-2-microglobulin; CDABP0092; HDCMA22P; BBM1; BBM.1; B2M recombinant antibody
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG kappa
Clone Number
BBM.1
Specificity
Binds human beta-2-microglobulin, a component of MHC class I molecules, which are present on all nucleated cells (excludes red blood cells) and involved in the presentation of peptide antigens to the immune system.
Purity/Purification
Protein A Affinity Purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Sequence Length
119
Applicable Applications for B2M recombinant antibody
IHC (Immunohistochemistry), WB (Western Blot), IF (Immunofluorescence)
Antibody Type
Recombinant Antibody
Antibody Species
Rabbit
Original Species Name
Mouse
Immunogen
Molt 4, a human T cell line.
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Buffer Composition
PBS only with 0.02% Proclin 300.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
WB (Western Blot)
(Western Blot using anti-Beta-2 microglobulin antibody BBM.1 HeLa cell lysate (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of BBM.1 (AAA72020) at 0.03 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Beta-2 microglobulin is 13.7 kDa. AAA72020 successfully detected human Beta-2 microglobulin in both HeLa cell extract and human duodenum samples.)
WB (Western Blot)
(Western Blot using anti-Beta-2 microglobulin antibody BBM.1 HeLa cell lysate (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of BBM.1 (AAA72020) at 0.03 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Beta-2 microglobulin is 13.7 kDa. AAA72020 successfully detected human Beta-2 microglobulin in both HeLa cell extract and human duodenum samples.)
IF (Immunofluorescence)
(Immunofluoresence staining of fixed HeLa cells with anti-Beta-2 microglobulin antibody BBM.1 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of BBM.1 (AAA72020) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72020, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluoresence staining of fixed HeLa cells with anti-Beta-2 microglobulin antibody BBM.1 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of BBM.1 (AAA72020) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72020, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow-cytometry using the anti-Beta-2 microglobulin antibody BBM.1 HeLa cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of BBM.1 (AAA72020, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flow-cytometry using the anti-Beta-2 microglobulin antibody BBM.1 HeLa cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of BBM.1 (AAA72020, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.)
Related Product Information for B2M recombinant antibody
Recombinant monoclonal antibody to Beta-2 microglobulin. Manufactured using the recombinant platform with variable regions (i.e. specificity) from the hybridoma BBM.1.
Product Categories/Family for B2M recombinant antibody
References
Brodsky FM, Bodmer WF, Parham P. Characterization of a monoclonal anti-beta-2-microglobulin antibody and its use in the genetic and biochemical analysis of major histocompatibility antigens. Eur J Immunol. 1979 Jul;9(7):536-45. PMID:91522
Original Published Journal: Eur J Immunol. 1979 Jul;9(7):536-45.
Original Published PMID: 14675065
Publication Note: Describes the generation of BBM.1 and characterization of its specific binding to bet 2-microglobulin.
Original Published Journal: Eur J Immunol. 1979 Jul;9(7):536-45.
Original Published PMID: 14675065
Publication Note: Describes the generation of BBM.1 and characterization of its specific binding to bet 2-microglobulin.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Beta-2-microglobulin
NCBI Official Synonym Full Names
beta-2-microglobulin
NCBI Official Symbol
B2M
NCBI Official Synonym Symbols
IMD43
NCBI Protein Information
beta-2-microglobulin
UniProt Protein Name
Beta-2-microglobulin
UniProt Gene Name
B2M
UniProt Entry Name
B2MG_HUMAN
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Product Notes
The B2M b2m (Catalog #AAA72020) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Beta-2 Microglobulin [BBM.1] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Beta-2 Microglobulin can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), IF (Immunofluorescence). Researchers should empirically determine the suitability of the B2M b2m for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Beta-2 Microglobulin, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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