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WB (Western Blot)
(The HeLa cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: ACTB antibody 1:1000Lane 2.: ACTB antibody 1:3000Lane 3.: ACTB antibody 1:5000Lane 4.: ACTB antibody 1:10000)
Mouse anti-Human beta-Actin Monoclonal Antibody | anti-ACTB antibody
Human beta-Actin antibody
Gene Names
ACTB; BRWS1; PS1TP5BP1
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Western Blot, ELISA
Purity
By protein-A affinity chromatography
Synonyms
beta-Actin, Antibody; Human beta-Actin antibody; Actin; cytoplasmic 1; Beta-actin; anti-ACTB antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1 kappa
Clone Number
AT4G6
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In Phosphate-Buffered Saline (pH7.4) with 0.02% Sodium Azide, 10% glycerol
Concentration
0.2mg/ml (determined by BCA assay) (varies by lot)
Applicable Applications for anti-ACTB antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA
Immunogen
Recombinant human ACTB (1-375aa) purified from E Coli
Conjugation
Unconjugated
Preparation and Storage
Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.
WB (Western Blot)
(The HeLa cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: ACTB antibody 1:1000Lane 2.: ACTB antibody 1:3000Lane 3.: ACTB antibody 1:5000Lane 4.: ACTB antibody 1:10000)
WB (Western Blot)
(The HeLa cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: ACTB antibody 1:1000Lane 2.: ACTB antibody 1:3000Lane 3.: ACTB antibody 1:5000Lane 4.: ACTB antibody 1:10000)
WB (Western Blot)
(The mouse tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Kidney tissue lysateLane 2.: Liver tissue lysateLane 3.: Spleen tissue lysateLane 4.: Bone marrow tissue lysateLane 5.: Stomach tissue lysate)
WB (Western Blot)
(The mouse tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Kidney tissue lysateLane 2.: Liver tissue lysateLane 3.: Spleen tissue lysateLane 4.: Bone marrow tissue lysateLane 5.: Stomach tissue lysate)
WB (Western Blot)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: Jurkat cell lysateLane 3.: PC3 cell lysateLane 4.: HepG2 cell lysateLane 5.: MCF7 cell lysateLane 6.: U87-MG cell lysateLane 7.: NIH-3T3 cell lysate)
WB (Western Blot)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: Jurkat cell lysateLane 3.: PC3 cell lysateLane 4.: HepG2 cell lysateLane 5.: MCF7 cell lysateLane 6.: U87-MG cell lysateLane 7.: NIH-3T3 cell lysate)
IF (Immunofluorescence)
(ICC/IF analysis of ACTB in HeLa cells. The cell was stained with ATGA0570 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
IF (Immunofluorescence)
(ICC/IF analysis of ACTB in HeLa cells. The cell was stained with ATGA0570 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
Related Product Information for anti-ACTB antibody
Beta-actin (human gene and protein symbol ACTB/ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus.
Product Categories/Family for anti-ACTB antibody
References
Gunning PW., et al. (2015) Journal of Cell Science. 128(11): 2009-2019. Hanukoglu I., et al. (1983) Journal of Molecular Biology. 163(4): 673-8.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
41,737 Da
NCBI Official Full Name
actin, cytoplasmic 1
NCBI Official Synonym Full Names
actin beta
NCBI Official Symbol
ACTB
NCBI Official Synonym Symbols
BRWS1; PS1TP5BP1
NCBI Protein Information
actin, cytoplasmic 1
UniProt Protein Name
Actin, cytoplasmic 1
UniProt Gene Name
ACTB
UniProt Entry Name
ACTB_HUMAN
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Product Notes
The ACTB actb (Catalog #AAA48358) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Human beta-Actin antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's beta-Actin can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the ACTB actb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "beta-Actin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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