Beta-Actin Recombinant Antibody | ACTB recombinant antibody

Beta-Actin Loading Control Antibody, Chimeric Rabbit MAb

Cat. Num. AAA259482

• Reactivity: Human
• Applications: Immunoprecipitation, Western Blot
• Purity: Protein A
• Synonyms: Beta-Actin, Recombinant Antibody; Beta-Actin Loading Control Antibody, Chimeric Rabbit MAb; actin, beta; Anti-BKRNS Antibody; Anti-BNS Antibody; Anti-BRWS1 Antibody; Anti-CSMH Antibody; Anti-DDS1 Antibody; Anti-PS1TP5BP1 Antibody; Anti-THC8 Antibody; ACTB recombinant antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 10
• Specificity: Human beta-Actin
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for ACTB recombinant antibody: IP (Immunoprecipitation), WB (Western Blot)
• Immunogen: A synthetic peptide corresponding to the N-terminus of the Human beta Actin/ACTB
• Preparation: It is a chimeric monoclonal antibody combining the constant domains of the rabbit IgG molecule with mouse variable regions. The variable region was obtained from a mouse immunized with A synthetic peptide corresponding to the N-terminus of the Human beta Actin/ACTB. The antibody was produced using recombinant antibody technology.
• Preparation and Storage: This antibody can be stored at 2 degree C-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.; This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

IP (Immunoprecipitation)

(Actin was immunoprecipitated using: Lane A:0.5 mg HeLa Whole Cell Lysate Lane B:0.5 mg Jurkat Whole Cell Lysate Lane C:0.5 mg MCF7 Whole Cell Lysate Lane D:0.5 mg A431 Whole Cell Lysate 4 uL anti-Actin rabbit monoclonal antibody and 60ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-Actin rabbit monoclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 43 kDa Observed band size :44 kDa)

WB (Western Blot)

(Anti-beta-Actin rabbit monoclonal antibody at 1:20000 dilutionLane A: Jurkat Whole Cell LysateLane B: HeLa Whole Cell LysateLane C: HepG2 Whole Cell LysateLane D: A549 Whole Cell LysateLane E: Mouse brain tissue lysateLane F: Mouse skeletal muscle tissue lysateLane G: Mouse kidney tissue lysateLane H: Rat brain tissue lysateLane I: Rat lung tissue lysateLane J: Rat kidney tissue lysateLysates/proteins at 30ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique. Performed under reducing conditions.Predicted band size:42 kDaObserved band size:42 kDa)

Related Product Information for ACTB recombinant antibody

ACTB (Actin Beta) is a Protein Coding gene. Diseases associated with ACTB include Dystonia, Juvenile-Onset and Baraitser-Winter Syndrome 1. Among its related pathways are Salivary secretion and Development Slit-Robo signaling. Gene Ontology (GO) annotations related to this gene include identical protein binding and RNA polymerase II proximal promoter sequence-specific DNA binding. An important paralog of this gene is ACTG1. TUBB, HPRT andACTBwere the most stably expressed genes for all analysis groups across meningioma and non-pathological meningeal tissue combined. ACTB, a significant upregulated gene in abdominal aortic aneurysm samples, could be regulated by CLIC4, which was significantly enriched in cell motions. Dystonia-deafness syndrome is a well-known clinical entity, with sensorineural deafness typically manifesting earlier than dystonia. ACTB p.Arg183Trp heterozygosity has been reported in six patients to cause combined infant-onset deafness and dystonia manifesting in adolescence or young adulthood.