Rabbit beta-Catenin Monoclonal Antibody | anti-CTNNB1 antibody

[KO Validated] beta-Catenin Rabbit mAb

Cat. Num. AAA28382

• Gene Names: CTNNB1; CTNNB; MRD19; armadillo
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Affinity purification
• Synonyms: beta-Catenin, Antibody; [KO Validated] beta-Catenin Rabbit mAb; CTNNB; MRD19; armadillo; beta Catenin; CTNNB1; anti-CTNNB1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.

• Applicable Applications for anti-CTNNB1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:50-1:200; IP: 1:50-1:200; Customer Validation:; WB: Human, Rat, Mouse; IF: Human; IHC: Human
• Immunogen: A synthesized peptide derived from human beta Catenin.
• Cellular Location: Cell junction, Cell membrane, Cytoplasm, Nucleus, adherens junction, centrosome, cytoskeleton, microtubule organizing center, spindle pole
• Positive Samples: HeLa, Mouse brain, 293T
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

IP (Immunoprecipitation)

(Immunoprecipitation analysis of 600ug extracts of Mouse brain using 3ug β-Catenin antibody . Western blot was performed from the immunoprecipitate using β-Catenin at a dilition of 1:1000.)

IF (Immunofluorescence)

(Immunofluorescence analysis of C6 cells using β-Catenin antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded Human pancreatic ductal carcinoma using [KO Validated] β-Catenin Rabbit mAb at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry)

(Immunohistochemistry of paraffin-embedded human lung squamous carcinoma tissue using [KO Validated] β-Catenin Rabbit mAb at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded Human Invasive ductal carcinoma of breast using [KO Validated] β-Catenin Rabbit mAb at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded Human cervical squamous cell carcinoma using [KO Validated] β-Catenin Rabbit mAb at dilution of 1:100 (40x lens).)

WB (Western Blot)

(Western blot analysis of extracts from normal (control) and β-Catenin knockout (KO) 293T cells, using β-Catenin antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)

WB (Western Blot)

(Western blot analysis of extracts of Mouse brain, using β-Catenin antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)

WB (Western Blot)

(Western blot analysis of extracts of HeLa cells, using β-Catenin antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)

Related Product Information for anti-CTNNB1 antibody

Background: The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016]

Product Categories/Family for anti-CTNNB1 antibody

Epigenetics & Nuclear Signaling; Transcription Factors; Research; Protein phosphorylation; Research; Cancer; Invasion and Metastasis; Research; Signal Transduction; ErbB-HER Signaling Pathway; Research; Cell Biology & Developmental Biology; Cell Adhesion;

NCBI and Uniprot Product Information

• NCBI GI #: 148233338
• NCBI GeneID: 1499
• NCBI Accession #: NP_001091679.1
• NCBI GenBank Nucleotide #: NM_001098209.1
• UniProt Accession #: P35222; Q8NEW9; Q8NI94; Q9H391; A8K1L7
• Molecular Weight: 85,497 Da
• NCBI Official Full Name: catenin beta-1
• NCBI Official Synonym Full Names: catenin (cadherin-associated protein), beta 1, 88kDa
• NCBI Official Symbol: CTNNB1
• NCBI Official Synonym Symbols: CTNNB; MRD19; armadillo
• NCBI Protein Information: catenin beta-1
• UniProt Protein Name: Catenin beta-1
• UniProt Gene Name: CTNNB1
• UniProt Synonym Gene Names: CTNNB
• UniProt Entry Name: CTNB1_HUMAN