Mouse beta-Tubulin Monoclonal Antibody | anti-TUBB antibody

beta-Tubulin

Cat. Num. AAA76724

• Gene Names: TUBB; M40; TUBB1; TUBB5; OK/SW-cl.56
• Reactivity: Human, Mouse, Rat, Primate
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot, Immunocytochemistry
• Synonyms: beta-Tubulin, Antibody; beta-Tubulin; anti-TUBB antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat, Primate
• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Form/Format: Liquid. Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3

• Applicable Applications for anti-TUBB antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ICC (Immunocytochemistry)
• Immunogen: Pig brain tubulin preparation
• Preparation and Storage: Antibody can also be aliquotted and stored frozen at -20 degree C in a manual defrost freezer for sixmonths without detectable loss of activity. The antibody can be stored at 2 degree - 8 degree C for 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

WB (Western Blot)

(Western blot analysis of whole tissue or cell lysates using mouse mAb to beta-tubulin, AAA76724, dilution 1:10,000 in green: [1] protein standard (red), [2] adult rat brain, [3] adult mouse brain, [4] NIH-3T3 cells, [5] HEK293 cells, [6] HeLa cells, [7] SH-SY5Y cells. The strong clean band with apparent SDS-PAGE molecular weight of ~50kDa corresponds to the beta-tubulin proteins.)

IF (Immunofluorescence)

(Immunofluorescent analysis of HeLa cells stained with mouse mAb to beta-tubulin, AAA76724, dilution 1:5,000 in green, and costained with chicken pAb to the nucleic acid binding protein FOX2 in red. Blue is DAPI staining of nuclear DNA. AAA76724 antibody produces strong staining of microtubules in the cytoplasm, while the FOX2 antibody labels the nuclei of HeLa cells.)

Related Product Information for anti-TUBB antibody

Tubulins are a major class of cytoskeletal proteins and divided into five distinct classes, namely alpha, beta, gamma, delta and epsilon. The most abundant members of this family are the alpha and beta-tubulins which are the major components of cytoplasmic microtubules. The various subunits have molecular weights of approximately 50kDa and are 50% identical to one another at the protein sequence level. Microtubules are involved in a number of essential cellular functions including the maintenance of cell shape, vesicle and organelle transport, cell motility, cell signaling, meiosis and mitosis. The important role of microtubules in forming the mitotic spindle during cell division makes them a desirable target for the development of therapeutic agents directed against rapidly dividing cancer cells.

This antibody was raised against tubulin purified from pig brain. Antibodies to beta-tubulin are widely used as loading controls in western blotting experiments as a standard by which the levels of other proteins may be measured.

NCBI and Uniprot Product Information

• NCBI GI #: 29788785
• NCBI GeneID: 203068
• NCBI Accession #: NP_821133.1
• NCBI GenBank Nucleotide #: NM_178014.2
• UniProt Accession #: P07437; P05218; Q8WUC1; Q9CY33
• Molecular Weight: 444
• NCBI Official Full Name: tubulin beta chain
• NCBI Official Synonym Full Names: tubulin, beta class I
• NCBI Official Symbol: TUBB
• NCBI Official Synonym Symbols: M40; TUBB1; TUBB5; OK/SW-cl.56
• NCBI Protein Information: tubulin beta chain; beta1-tubulin; beta 5-tubulin; beta-4 tubulin; beta Ib tubulin; class I beta-tubulin; tubulin beta-1 chain; tubulin beta-5 chain; tubulin beta polypeptide; tubulin, beta polypeptide
• UniProt Protein Name: Tubulin beta chain
• UniProt Gene Name: TUBB
• UniProt Synonym Gene Names: TUBB5
• UniProt Entry Name: TBB5_HUMAN