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WB (Western Blot)
(Detection of mouse BRCA1 by western blot. Samples: Whole cell lysate (50 ug) from J774A1 and CT26 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRCA1 recombinant monoclonal antibody (AAA213708 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
BRCA1 recombinant antibody
Rabbit anti-BRCA1 Recombinant Monoclonal Antibody [BLR319M]
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
BRCA1, Recombinant Antibody; Rabbit anti-BRCA1 Recombinant Monoclonal Antibody [BLR319M]; breast cancer associated 1; breast cancer type 1 susceptibility protein homolog; RING-type E3 ubiquitin transferase BRCA1; Breast cancer type 1 susceptibility protein homolog; breast cancer 1, early onset; BRCA1 recombinant antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Clone Number
BLR319M
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Phosphate Buffered Saline (PBS) with 0.09% Sodium Azide, BSA-Free
Concentration
1000ug/ml (varies by lot)
Applicable Applications for BRCA1 recombinant antibody
FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 525 and 575
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of mouse BRCA1 by western blot. Samples: Whole cell lysate (50 ug) from J774A1 and CT26 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRCA1 recombinant monoclonal antibody (AAA213708 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot)
(Detection of mouse BRCA1 by western blot. Samples: Whole cell lysate (50 ug) from J774A1 and CT26 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRCA1 recombinant monoclonal antibody (AAA213708 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot)
(Detection of mouse BRCA1 by western blot. Samples: Whole cell lysate (50 ug) from mouse fibroblast BRCA1 delta11/delta11(S12) and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRCA1 recombinant monoclonal antibody (AAA213708 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot)
(Detection of mouse BRCA1 by western blot. Samples: Whole cell lysate (50 ug) from mouse fibroblast BRCA1 delta11/delta11(S12) and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRCA1 recombinant monoclonal antibody (AAA213708 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
IP (Immunoprecipitation)
(Detection of mouse BRCA1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from J774A1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRCA1 recombinant monoclonal antibody (AAA213708 lot 1) used at 6 ul per reaction. For blotting immunoprecipitated BRCA1, AAA213708 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of mouse BRCA1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from J774A1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRCA1 recombinant monoclonal antibody (AAA213708 lot 1) used at 6 ul per reaction. For blotting immunoprecipitated BRCA1, AAA213708 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
FCM/FACS (Flow Cytometry)
(Detection of mouse BRCA1 (shaded) in NIH3T3 cells by flow cytometry. Antibody: Rabbit anti-BRCA1 recombinant monoclonal antibody (AAA213708) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM/FACS (Flow Cytometry)
(Detection of mouse BRCA1 (shaded) in NIH3T3 cells by flow cytometry. Antibody: Rabbit anti-BRCA1 recombinant monoclonal antibody (AAA213708) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
Related Product Information for BRCA1 recombinant antibody
BRCA1 was identified as a gene with germline mutations that predisposes women to breast and ovarian cancer. The BRCA1 tumor suppressor is a large protein that has been demonstrated to interact with a multitude of proteins involved in the DNA damage response and cell cycle checkpoints to maintain genomic integrity. BRCA1 functions by regulating the activities of its interacting proteins which mediate processes such as gene transcription, gene repression, protein phosphorylation, and ubiquitination.
Product Categories/Family for BRCA1 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
198,795 Da
NCBI Official Full Name
breast cancer type 1 susceptibility protein homolog
NCBI Official Synonym Full Names
breast cancer 1, early onset
NCBI Official Symbol
Brca1
NCBI Protein Information
breast cancer type 1 susceptibility protein homolog
UniProt Protein Name
Breast cancer type 1 susceptibility protein homolog
UniProt Gene Name
Brca1
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Product Notes
The BRCA1 brca1 (Catalog #AAA213708) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-BRCA1 Recombinant Monoclonal Antibody [BLR319M] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's BRCA1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the BRCA1 brca1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BRCA1, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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