FCM/FACS (Flow Cytometry)
(Overlay histogram showing Hela cells stained with (red line) at 1?50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1ug/1*106cells) for 1 h at 4?.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4?. Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
BUB1B recombinant antibody
BUB1B Antibody
Gene Names
BUB1B; MVA1; SSK1; BUBR1; Bub1A; MAD3L; hBUBR1; BUB1beta
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot, ELISA
Purity
Affinity-chromatography
Synonyms
BUB1B, Recombinant Antibody; BUB1B Antibody; Mitotic checkpoint serine/threonine-protein kinase BUB1 beta (EC 2.7.11.1) (MAD3/BUB1-related protein kinase) (hBUBR1) (Mitotic checkpoint kinase MAD3L) (Protein SSK1); BUB1B; BUBR1 MAD3L SSK1; BUB1B recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
7H4
Purity/Purification
Affinity-chromatography
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, PH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applicable Applications for BUB1B recombinant antibody
FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA
Antibody Type
Recombinant Antibody
Conjugation
Non-conjugated
Immunogen
A synthesized peptide derived from human BubR1
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
FCM/FACS (Flow Cytometry)
(Overlay histogram showing Hela cells stained with (red line) at 1?50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1ug/1*106cells) for 1 h at 4?.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4?. Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
FCM/FACS (Flow Cytometry)
(Overlay histogram showing Hela cells stained with (red line) at 1?50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1ug/1*106cells) for 1 h at 4?.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4?. Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
WB (Western Blot)
(Western BlotPositive WB detected in: K562 whole cell lysate, U-251 whole cell lysateAll lanes: BubR1 antibody at 1:1000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 120, 106, 122 kDaObserved band size: 125 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in: K562 whole cell lysate, U-251 whole cell lysateAll lanes: BubR1 antibody at 1:1000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 120, 106, 122 kDaObserved band size: 125 kDa)
Related Product Information for BUB1B recombinant antibody
Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression.
Product Categories/Family for BUB1B recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
119,545 Da
NCBI Official Full Name
mitotic checkpoint serine/threonine-protein kinase BUB1 beta
NCBI Official Synonym Full Names
BUB1 mitotic checkpoint serine/threonine kinase B
NCBI Official Symbol
BUB1B
NCBI Official Synonym Symbols
MVA1; SSK1; BUBR1; Bub1A; MAD3L; hBUBR1; BUB1beta
NCBI Protein Information
mitotic checkpoint serine/threonine-protein kinase BUB1 beta; mitotic checkpoint kinase MAD3L; MAD3/BUB1-related protein kinase; budding uninhibited by benzimidazoles 1 homolog beta
UniProt Protein Name
Mitotic checkpoint serine/threonine-protein kinase BUB1 beta
UniProt Gene Name
BUB1B
UniProt Synonym Gene Names
BUBR1; MAD3L; SSK1; hBUBR1
UniProt Entry Name
BUB1B_HUMAN
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Product Notes
The BUB1B bub1b (Catalog #AAA243880) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BUB1B Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BUB1B can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the BUB1B bub1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BUB1B, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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