Application Data
(Fig.2: SDS-PAGE analysis of purified BCI-3E7 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified BCI-3C7 antibody. Proteins were separated by SDSPAGE and stained with RAPID StainTM Reagent.)
Rat anti-Human c-Met Monoclonal Antibody | anti-MET antibody
Anti-c Met monoclonal antibody
Gene Names
MET; HGFR; AUTS9; RCCP2; c-Met
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay
Purity
Protein G
Synonyms
c-Met, Antibody; Anti-c Met monoclonal antibody; anti-MET antibody
Host
Rat
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b, kappa
Clone Number
BCI-3E7
Specificity
anti-human c-Met
Purity/Purification
Protein G
Form/Format
Purified
Sequence Length
1390
Applicable Applications for anti-MET antibody
ELISA (Competitive ELISA), FCM/FACS (Flow Cytometry)
Buffer
phosphate buffered saline, pH 7.2
Immunogen
genetic immunisation with cDNA encoding human c-Met
Selection
based on recognition of the complete native protein expressed on transfected mammalian cells
Immunogen
cDNA
Preparation and Storage
Short term: 2 degree C - 8 degree C; long term: -20 degree C (avoid repeated freezing and thawing)
Application Data
(Fig.2: SDS-PAGE analysis of purified BCI-3E7 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified BCI-3C7 antibody. Proteins were separated by SDSPAGE and stained with RAPID StainTM Reagent.)
Application Data
(Fig.2: SDS-PAGE analysis of purified BCI-3E7 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified BCI-3C7 antibody. Proteins were separated by SDSPAGE and stained with RAPID StainTM Reagent.)
Application Data
(Fig.1: FACS analysis of BOSC23 cells using BCI-3E7 Cat.# GM- 1005. BOSC23 cells were transiently transfected with an expression vector encoding either c-Met (red curve) or an irrelevant protein (control transfectant: black curve). Binding of BCI-3E7 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with c-Met transfected cells.)
Application Data
(Fig.1: FACS analysis of BOSC23 cells using BCI-3E7 Cat.# GM- 1005. BOSC23 cells were transiently transfected with an expression vector encoding either c-Met (red curve) or an irrelevant protein (control transfectant: black curve). Binding of BCI-3E7 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with c-Met transfected cells.)
Related Product Information for anti-MET antibody
Background: c-MET is a receptor tyrosine kinase that is activated upon Hepatocyte Growth Factor (HGF) binding. c-MET is produced as a single-chain precursor and plays en essential role in embryonic development and wound healing. It belongs to the class of Singlepass type I membrane proteins and it is normally expressed by cells of epithelial origin while the expression of HGF is restricted to cells of mesenchymal origin in an almost perfect paracrine paradigm. Overexpression of c-MET can be found in several kinds of tumor progression.
References
Bottaro DP, Rubin JS, Faletto DL, Chan AM, Kmiecik TE, Vande Woude GF, Aaronson SA (1991). Identification of the hepatocyte growth factor receptor as the met proto-oncogene product. Science 251 (4995): 802–4
Galland F, Stefanova M, Lafage M, Birnbaum D (1992). Localization of the 5' end of the MCF2 oncogene to human chromosome 15q15----q23. Cytogenet. Cell Genet. 60 (2): 114–6.
Cooper CS (1992). The met oncogene: from detection by transfection to transmembrane receptor for hepatocyte growth factor. Oncogene 7 (1): 3–7.
Galland F, Stefanova M, Lafage M, Birnbaum D (1992). Localization of the 5' end of the MCF2 oncogene to human chromosome 15q15----q23. Cytogenet. Cell Genet. 60 (2): 114–6.
Cooper CS (1992). The met oncogene: from detection by transfection to transmembrane receptor for hepatocyte growth factor. Oncogene 7 (1): 3–7.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
85,745 Da
NCBI Official Full Name
hepatocyte growth factor receptor isoform b
NCBI Official Synonym Full Names
MET proto-oncogene, receptor tyrosine kinase
NCBI Official Symbol
MET
NCBI Official Synonym Symbols
HGFR; AUTS9; RCCP2; c-Met
NCBI Protein Information
hepatocyte growth factor receptor; SF receptor; HGF receptor; HGF/SF receptor; proto-oncogene c-Met; scatter factor receptor; tyrosine-protein kinase Met; met proto-oncogene tyrosine kinase
UniProt Gene Name
MET
UniProt Synonym Gene Names
HGF receptor; SF receptor
UniProt Entry Name
MET_HUMAN
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Product Notes
The MET met (Catalog #AAA71473) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-c Met monoclonal antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's c-Met can be used in a range of immunoassay formats including, but not limited to, ELISA (Competitive ELISA), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the MET met for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "c-Met, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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