IF (Immunofluorescence)
(ICC/IF analysis of C1QBP in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human C1QBP antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Mouse anti-Human C1QBP Monoclonal Antibody | anti-C1QBP antibody
C1QBP antibody
Gene Names
C1QBP; p32; HABP1; gC1qR; GC1QBP; SF2p32; gC1Q-R; COXPD33
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Flow Cytometry, Functional Assay, Western Blot, ELISA
Purity
By protein-A affinity chromatography
Synonyms
C1QBP, Antibody; C1QBP antibody; Complement component 1 q subcomponent binding protein; p33; HABP1; gC1qR; GC1QBP; SF2p32,Complement component 1 q subcomponent binding protein; C1QBP; Complement component 1; q subcomponent binding protein C1q globular domain binding protein; Complement component 1 Q subcomponent binding protein mitochondrial; GC1Q R; GC1q R protein; GC1QR; Glycoprotein gC1qBP; HABP 1; Hyaluronan binding protein 1; Mitochondrial matrix protein p32; p32; Pre mrna splicing factor SF2 P32 subunit precursor; Splicing factor SF2 associated protein.; anti-C1QBP antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
AT1G7
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
282
Applicable Applications for anti-C1QBP antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA
Antigen Species
Human
Immunogen
Recombinant human C1QBP (74-282aa) purified from E Coli.
Clone Note
Anti-human C1QBP mAb, clone AT1G7, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human C1QBP protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
IF (Immunofluorescence)
(ICC/IF analysis of C1QBP in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human C1QBP antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
IF (Immunofluorescence)
(ICC/IF analysis of C1QBP in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human C1QBP antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot)
(The recombinant protein (50ng) and cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human C1QBP antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : Recombinant protein Lane 2 : HeLa cell lysate Lane 3 : Jurkat cell lysate Lane 4 : MCF7 cell lysate Lane 5 : Ramos cell lysate Lane 6 : 293T cell lysate Lane 7 : A549 cell lysate)
WB (Western Blot)
(The recombinant protein (50ng) and cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human C1QBP antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : Recombinant protein Lane 2 : HeLa cell lysate Lane 3 : Jurkat cell lysate Lane 4 : MCF7 cell lysate Lane 5 : Ramos cell lysate Lane 6 : 293T cell lysate Lane 7 : A549 cell lysate)
Related Product Information for anti-C1QBP antibody
Complement component 1 Q subcomponent-binding protein, mitochondrial is a protein that in humans is encoded by the C1QBP gene. The human complement subcomponent C1q associates with C1r and C1s in order to yield the first component of the serum complement system. The protein encoded by this gene is known to bind to the globular heads of C1q molecules and inhibit C1 activation. This protein has also been identified as the p32 subunit of pre-mRNA splicing factor SF2, as well as a hyaluronic acid-binding protein.
Product Categories/Family for anti-C1QBP antibody
References
Deb TB, et al. (1996) J Biol Chem 271 (4):2206-12. ; Ghebrehiwet B, et al. (1994) J Exp Med 179 (6):1809-21.; Entrez Gene: C1QBP complement component 1, q subcomponent binding protein.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31,362 Da
NCBI Official Full Name
complement component 1 Q subcomponent-binding protein, mitochondrial
NCBI Official Synonym Full Names
complement C1q binding protein
NCBI Official Symbol
C1QBP
NCBI Official Synonym Symbols
p32; HABP1; gC1qR; GC1QBP; SF2p32; gC1Q-R; COXPD33
NCBI Protein Information
complement component 1 Q subcomponent-binding protein, mitochondrial
UniProt Protein Name
Complement component 1 Q subcomponent-binding protein, mitochondrial
UniProt Gene Name
C1QBP
UniProt Synonym Gene Names
GC1QBP; HABP1; SF2P32; C1qBP
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Product Notes
The C1QBP c1qbp (Catalog #AAA48148) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The C1QBP antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's C1QBP can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the C1QBP c1qbp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "C1QBP, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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