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IF (Immunofluorescence)
(AAA324634 staining A549 cells by IF/ICC. The samples were fixedwith PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA324634) and mouse anti-betatubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L)Ab(Green) were used as the secondary antibody.)
Rabbit C1QBP Polyclonal Antibody | anti-C1QBP antibody
C1QBP Antibody
Gene Names
C1QBP; p32; HABP1; gC1qR; GC1QBP; SF2p32; gC1Q-R; COXPD33; SF2AP32
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Peptide affinity purification
Synonyms
C1QBP, Antibody; C1QBP Antibody; ASF/SF2 associated protein p32; C1q globular domain binding protein; C1qBP; C1QBP_HUMAN; Complement component 1 q subcomponent binding protein; Complement component 1 Q subcomponent binding protein mitochondrial; Complement component 1 Q subcomponent-binding protein, mitochondrial; GC1Q R; GC1q R protein; GC1q-R protein; GC1QBP; GC1QR; globular domain of, C1q, receptor for; Glycoprotein gC1qBP; HABP 1; HABP1; Hyaluronan binding protein 1; Hyaluronan-binding protein 1; Mitochondrial matrix protein p32; p32; p32 splicing factor; p33; Pre mrna splicing factor SF2 P32 subunit precursor; SF2p32; Splicing factor SF2 associated protein; anti-C1QBP antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
C1QBP Antibody detects endogenous levels of total C1QBP.
Purity/Purification
Peptide affinity purification
Form/Format
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-C1QBP antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human C1QBP, corresponding to a region within the internal amino acids.
Preparation and Storage
Store at -20°C
IF (Immunofluorescence)
(AAA324634 staining A549 cells by IF/ICC. The samples were fixedwith PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA324634) and mouse anti-betatubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L)Ab(Green) were used as the secondary antibody.)
IF (Immunofluorescence)
(AAA324634 staining A549 cells by IF/ICC. The samples were fixedwith PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA324634) and mouse anti-betatubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L)Ab(Green) were used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of extracts from mouse brain, using C1QBP antibody.)
WB (Western Blot)
(Western blot analysis of extracts from mouse brain, using C1QBP antibody.)
IHC (Immunohiostchemistry)
(AAA324634 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohiostchemistry)
(AAA324634 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot)
(Western blot analysis of extracts from Jurkat cells, using C1QBP antibody.)
WB (Western Blot)
(Western blot analysis of extracts from Jurkat cells, using C1QBP antibody.)
Related Product Information for anti-C1QBP antibody
Is believed to be a multifunctional and multicompartmental protein involved in inflammation and infection processes, ribosome biogenesis, protein synthesis in mitochondria, regulation of apoptosis, transcriptional regulation and pre-mRNA splicing. At the cell surface is thought to act as an endothelial receptor for plasma proteins of the complement and kallikrein-kinin cascades. Putative receptor for C1q; specifically binds to the globular "heads" of C1q thus inhibiting C1; may perform the receptor function through a complex with C1qR/CD93. In complex with cytokeratin-1/KRT1 is a high affinity receptor for kininogen-1/HMWK. Can also bind other plasma proteins, such as coagulation factor XII leading to its autoactivation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
32 KD
NCBI Official Full Name
complement component 1 Q subcomponent-binding protein, mitochondrial
NCBI Official Synonym Full Names
complement C1q binding protein
NCBI Official Symbol
C1QBP
NCBI Official Synonym Symbols
p32; HABP1; gC1qR; GC1QBP; SF2p32; gC1Q-R; COXPD33; SF2AP32
NCBI Protein Information
complement component 1 Q subcomponent-binding protein, mitochondrial
UniProt Protein Name
Complement component 1 Q subcomponent-binding protein, mitochondrial
UniProt Gene Name
C1QBP
UniProt Synonym Gene Names
GC1QBP; HABP1; SF2P32; C1qBP; SF2AP32
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Product Notes
The C1QBP c1qbp (Catalog #AAA324634) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The C1QBP Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's C1QBP can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the C1QBP c1qbp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "C1QBP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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