FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Human CXADR expression on HT-29 cells. Cells were stained with purified anti-Human CXADR, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Rabbit anti-Human CAR/CXADR Monoclonal Antibody | anti-CXADR antibody
Recombinant Anti-CAR/CXADR Antibody, Rabbit Monoclonal
Gene Names
CXADR; CAR; HCAR; CAR4/6
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Flow Cytometry, Functional Assay, ELISA
Synonyms
CAR/CXADR, Antibody; Recombinant Anti-CAR/CXADR Antibody, Rabbit Monoclonal; CXADR/CAR Antibody, Rabbit MAb; coxsackie virus and adenovirus receptor; Anti-CAR Antibody; Anti-CAR4/6 Antibody; Anti-CXADR Antibody; Anti-HCAR Antibody; anti-CXADR antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
271
Specificity
Human CXADR/CAR
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CXADR antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), ELISA
Immunogen
Recombinant Human CXADR/CAR protein
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human CXADR/CAR (rh CXADR/CAR; MBS8120490; NP_001329.1; Met1-Gly237)
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Human CXADR expression on HT-29 cells. Cells were stained with purified anti-Human CXADR, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Human CXADR expression on HT-29 cells. Cells were stained with purified anti-Human CXADR, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
IF (Immunofluorescence)
(Immunofluorescence staining of Human CXADR in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human CXADR monoclonal antibody (1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cells membrane.)
IF (Immunofluorescence)
(Immunofluorescence staining of Human CXADR in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human CXADR monoclonal antibody (1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cells membrane.)
References
Tomko, R.P. et al., 1997, Proc. Natl. Acad. Sci. U.S.A. 94 (7): 3352-3356. van Raaij, M.J. et al., 2001, Structure. 8 (11): 1147-1155. Cohen, C.J. et al., 2001, J. Biol. Chem. 276 (27): 25392-25398. Carson, S.D. et al., 2002, Rev. Med. Virol. 11 (4): 219-226. Selinka, H.C. et al., 2004, Med. Microbiol. Immunol. 193 (2-3): 127-131. Philipson, L. et al., 2004, Curr. Top. Microbiol. Immunol. 273:87-111. Raschperger, E. et al., 2006, Exp. Cell Res. 312: 1566-1580.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
coxsackievirus and adenovirus receptor isoform 1
NCBI Official Synonym Full Names
coxsackie virus and adenovirus receptor
NCBI Official Symbol
CXADR
NCBI Official Synonym Symbols
CAR; HCAR; CAR4/6
NCBI Protein Information
coxsackievirus and adenovirus receptor; HCVADR; CVB3-binding protein; coxsackievirus B-adenovirus receptor; 46 kD coxsackievirus and adenovirus receptor (CAR) protein
UniProt Protein Name
Coxsackievirus and adenovirus receptor
UniProt Gene Name
CXADR
UniProt Synonym Gene Names
CAR; CAR; hCAR
UniProt Entry Name
CXAR_HUMAN
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Product Notes
The CXADR cxadr (Catalog #AAA255167) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-CAR/CXADR Antibody, Rabbit Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CAR/CXADR can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), ELISA. Researchers should empirically determine the suitability of the CXADR cxadr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CAR/CXADR, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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