IP (Immunoprecipitation)
(Mouse CA8 was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell LysateLane B:0.5 mg A549 Whole Cell Lysate2 uL anti-Mouse CA8 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse CA8 rabbit monoclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 33 kDaObserved band size: 37 kDa)
Rabbit anti-Mouse Carbonic Anhydrase 8/CA8 Monoclonal Antibody | anti-CA8 antibody
Recombinant Anti-Carbonic Anhydrase 8/CA8 Antibody, Rabbit Monoclonal
Gene Names
Car8; Ca8; wdl; Cals; Carp; Cals1; AW546993
Reactivity
Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Protein A
Synonyms
Carbonic Anhydrase 8/CA8, Antibody; Recombinant Anti-Carbonic Anhydrase 8/CA8 Antibody, Rabbit Monoclonal; Carbonic Anhydrase VIII/Car8 Antibody, Rabbit MAb; carbonic anhydrase VIII; Anti-AW546993 Antibody; Anti-Ca8 Antibody; Anti-Cals Antibody; Anti-Cals1 Antibody; Anti-Car8 Antibody; Anti-Carp Antibody; Anti-RP23-180H12.1 Antibody; Anti-wdl Antibody; anti-CA8 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
005
Specificity
Mouse Carbonic Anhydrase VIII/CA8
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CA8 antibody
IP (Immunoprecipitation), WB (Western Blot)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Mouse Carbonic Anhydrase VIII/CA8 protein
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse Carbonic Anhydrase VIII/CA8 (rM Carbonic Anhydrase VIII/CA8).
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(Mouse CA8 was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell LysateLane B:0.5 mg A549 Whole Cell Lysate2 uL anti-Mouse CA8 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse CA8 rabbit monoclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 33 kDaObserved band size: 37 kDa)
IP (Immunoprecipitation)
(Mouse CA8 was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell LysateLane B:0.5 mg A549 Whole Cell Lysate2 uL anti-Mouse CA8 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse CA8 rabbit monoclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 33 kDaObserved band size: 37 kDa)
WB (Western Blot)
(Anti-CA8 rabbit monoclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLane B: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:33 kDaObserved band size:36 kDa)
WB (Western Blot)
(Anti-CA8 rabbit monoclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLane B: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:33 kDaObserved band size:36 kDa)
Related Product Information for anti-CA8 antibody
The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase protein (CA) VIII, which is a member of the CA gene family, has been shown to have no catalytic CA activity and its biological function is still unknown. Increased expression of CA-RP VIII was observed in 78% of colorectal carcinomas. It suggested that CA-RP VIII plays a role in the process of invasion in colorectal cancer.
References
Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1):1-20.Miyaji E, et al. (2003) Overexpression of carbonic anhydrase-related protein VIII in human colorectal cancer. The Journal of Pathology. 201(1): 37-45.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,081 Da
NCBI Official Full Name
carbonic anhydrase-related protein
NCBI Official Synonym Full Names
carbonic anhydrase 8
NCBI Official Symbol
Car8
NCBI Official Synonym Symbols
Ca8; wdl; Cals; Carp; Cals1; AW546993
NCBI Protein Information
carbonic anhydrase-related protein
UniProt Protein Name
Carbonic anhydrase-related protein
UniProt Gene Name
Ca8
UniProt Synonym Gene Names
Cals; Cals1; Car8; Carp; CARP; CA-VIII
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Product Notes
The CA8 ca8 (Catalog #AAA257224) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-Carbonic Anhydrase 8/CA8 Antibody, Rabbit Monoclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Carbonic Anhydrase 8/CA8 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the CA8 ca8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Carbonic Anhydrase 8/CA8, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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