WB (Western Blot)
(Western Blot Analysis of HCT116 Cell Lysate using CAIX Monoclonal Antibody (CA9/781).)
Mouse anti-Human, Horse Carbonic Anhydrase IX Monoclonal Antibody | anti-CA9 antibody
Carbonic Anhydrase IX (Renal Cell Marker) Mouse Monoclonal Antibody
Gene Names
CA9; MN; CAIX
Reactivity
Human, Horse
Applications
Immunohistochemistry, Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
Synonyms
Carbonic Anhydrase IX, Antibody; Carbonic Anhydrase IX (Renal Cell Marker) Mouse Monoclonal Antibody; CA-IX, CA9, CAH9_HUMAN, CAIX, Carbonate dehydratase IX, Carbonic anhydrase 9, Carbonic anhydrase IX, Carbonic dehydratase, G250, Membrane antigen MN, P54/58N, pMW1, RCC-associated antigen G250, Renal cell carcinoma-associated antigen G250; anti-CA9 antibody
Host
Mouse
Reactivity
Human, Horse
Clonality
Monoclonal
Isotype
IgG2b, kappa
Clone Number
CA9/781
Specificity
Recognizes a glycoprotein of ~200kDa, identified as carbonic anhydrase IX (CAIX/gp200). Carbonic Anhydrases (CAs) are members of a large family of zinc metallo-enzymes that catalyze the reversible hydration of carbon dioxide. CAs are involved in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption and the formation of aqueous humor, cerebrospinal fluid, saliva and gastric juice. They show extensive diversity in distribution and in their subcellular localization. CA IX is specifically expressed in clear-cell renal carcinomas.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
459
Applicable Applications for anti-CA9 antibody
IHC (Immunohistochemistry), WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
Cellular Localization
Cell Surface and Cytoplasmic
Immunogen
Recombinant human CA9 protein
Hu-Chromosome Location
9p13.3
Positive Control
Normal kidney or renal cell carcinoma
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
WB (Western Blot)
(Western Blot Analysis of HCT116 Cell Lysate using CAIX Monoclonal Antibody (CA9/781).)
WB (Western Blot)
(Western Blot Analysis of HCT116 Cell Lysate using CAIX Monoclonal Antibody (CA9/781).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with CAIX Monoclonal Antibody (CA9/781).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with CAIX Monoclonal Antibody (CA9/781).)
References
Sly, W.S., et al. 1995. Human Carbonic Anhydrases and Carbonic Anhydrase deficiencies. Annu. Rev. Biochem. 64: 375-401
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
55kDa
NCBI Official Full Name
carbonic anhydrase 9
NCBI Official Synonym Full Names
carbonic anhydrase 9
NCBI Official Symbol
CA9
NCBI Official Synonym Symbols
MN; CAIX
NCBI Protein Information
carbonic anhydrase 9
UniProt Protein Name
Carbonic anhydrase 9
UniProt Gene Name
CA9
UniProt Synonym Gene Names
G250; MN; CA-IX; CAIX; RCC-associated antigen G250
UniProt Entry Name
CAH9_HUMAN
Similar Products
Product Notes
The CA9 ca9 (Catalog #AAA62798) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Carbonic Anhydrase IX (Renal Cell Marker) Mouse Monoclonal Antibody reacts with Human, Horse and may cross-react with other species as described in the data sheet. AAA Biotech's Carbonic Anhydrase IX can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CA9 ca9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Carbonic Anhydrase IX, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.