WB (Western Blot)
(Western Blot using anti-Cardiac Troponin I antibody scFv 180 Human heart lysate (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric mouse IgG1 version of scFv 180 at 0.001 ug/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The predicted running size for Cardiac Troponin I is 24.0 kDa. It successfully detected Cardiac Troponin I in human heart lysate.)
Cardiac Troponin I Recombinant Antibody | cTnI recombinant antibody
Anti-Cardiac Troponin I [scFv 180]
Gene Names
TNNI3; CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF
Reactivity
Human
Applications
Western Blot
Purity
Protein A Affinity Purified
Synonyms
Cardiac Troponin I, Recombinant Antibody; Anti-Cardiac Troponin I [scFv 180]; TNNI3; cTn-I; scFv 180; cTnI recombinant antibody
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG lambda
Clone Number
scFv 180
Specificity
This antibody binds specificially to the cardiac Troponin I Peptide KISASRKLQLKT. This epitope is 100% conserved among a great variety of mammalian species, including most monkeys, Squirrel, Hamster, Mouse, Rat, Rabbit, Whales, Mole-Rat, Antelope and several other mammals.
Purity/Purification
Protein A Affinity Purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Sequence Length
210
Applicable Applications for cTnI recombinant antibody
SPR (Surface Plasmon Resonance), WB (Western Blot)
Antibody Type
Recombinant Antibody
Antibody Species
Rabbit
Original Species Name
Chicken
Immunogen
Cardiac Troponin I peptide (KISASRKLQLKT).
Chimeric Use Statement
This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Chicken scFv format, for improved compatibility with existing reagents, assays and techniques.
Clone Note
Troponin I is the inhibitory subunit of troponin, the thin filament complex that confers calcium-sensitivity to muscle-actomyoisin ATPase activity. Cardiac Troponin I in the blood is also a marker for several heart muscle damages including myocardial infarction.
Buffer Composition
PBS only with 0.02% Proclin 300.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
WB (Western Blot)
(Western Blot using anti-Cardiac Troponin I antibody scFv 180 Human heart lysate (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric mouse IgG1 version of scFv 180 at 0.001 ug/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The predicted running size for Cardiac Troponin I is 24.0 kDa. It successfully detected Cardiac Troponin I in human heart lysate.)
WB (Western Blot)
(Western Blot using anti-Cardiac Troponin I antibody scFv 180 Human heart lysate (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric mouse IgG1 version of scFv 180 at 0.001 ug/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The predicted running size for Cardiac Troponin I is 24.0 kDa. It successfully detected Cardiac Troponin I in human heart lysate.)
IF (Immunofluorescence)
(Immunofluorescence staining of fixed HeLa cells with anti-Cardiac Troponin I antibody scFv 180 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of scFv 180 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of fixed HeLa cells with anti-Cardiac Troponin I antibody scFv 180 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of scFv 180 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.)
Related Product Information for cTnI recombinant antibody
Recombinant monoclonal antibody to Cardiac Troponin I. Manufactured using the recombinant platform with variable regions (i.e. specificity) from the scFv scFv 180.
Product Categories/Family for cTnI recombinant antibody
References
Conroy et al. Reconciling the structural attributes of avian antibodies. Journal of Biological Chemsitry 2014; 289(22):15384-15392 PMID:24737329
Original Published Journal: Journal of Biological Chemsitry 2014; 289(22):15384-15392
Original Published Journal: Journal of Biological Chemsitry 2014; 289(22):15384-15392
Publication Note: Describes the use of phage display libraries to generate chicken antibodies against human cardiac troponin and PSA with subsequent crystallisation.
Original Published Journal: Journal of Biological Chemsitry 2014; 289(22):15384-15392
Original Published Journal: Journal of Biological Chemsitry 2014; 289(22):15384-15392
Publication Note: Describes the use of phage display libraries to generate chicken antibodies against human cardiac troponin and PSA with subsequent crystallisation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
troponin I, cardiac muscle
NCBI Official Synonym Full Names
troponin I3, cardiac type
NCBI Official Symbol
TNNI3
NCBI Official Synonym Symbols
CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF
NCBI Protein Information
troponin I, cardiac muscle
UniProt Protein Name
Troponin I, cardiac muscle
UniProt Gene Name
TNNI3
UniProt Synonym Gene Names
TNNC1
UniProt Entry Name
TNNI3_HUMAN
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Product Notes
The cTnI tnni3 (Catalog #AAA72031) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Cardiac Troponin I [scFv 180] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cardiac Troponin I can be used in a range of immunoassay formats including, but not limited to, SPR (Surface Plasmon Resonance), WB (Western Blot). Researchers should empirically determine the suitability of the cTnI tnni3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cardiac Troponin I, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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