Mouse Catenin, beta, active Monoclonal Antibody | anti-CTNNB1 antibody

Catenin, beta, active (ABC) (BSA, Azide & Glycerol Free)

Cat. Num. AAA14699

• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: Purified by Protein G affinity chromatography.
• Synonyms: Catenin, beta, active, Antibody; Catenin, beta, active (ABC) (BSA, Azide & Glycerol Free); Anti -Catenin, beta, active (ABC) (BSA, Azide & Glycerol Free); anti-CTNNB1 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 3C196
• Specificity: Epitope corresponds to amino acid residues 36-44 (HSGATTTAP) of human beta-catenin, Mr 92kD. Specific for the active form of beta-catenin, nonphosphorylated on Ser37 and Thr41.1 A non-specific protein, Mr 130kD, was also detected when used at higher concentrations. Species Crossreactivity: Human, mouse and rat, NOT Drosophila.
• Purity/Purification: Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4 No stabilizing proteins added. No preservative added. Also available w/ azide.
• Concentration: 1mg/mL (varies by lot)

• Applicable Applications for anti-CTNNB1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in Immunohistochemistry, Western Blot, Immunocytochemistry and Flow Cytometry. Other applications not tested.; Immunohistochemisty (Paraffin): Detects b-Catenin in mouse embryo sections. Requires HIER using sodium citrate buffer pH6.0 Immunocytochemistry: Shows positive staining in LiCl-treated 293T cells fixed with methanol.; Western Blot: 0.2-2ug/ml; A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with AAA14699. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system.; Flow Cytometry: 1ug/ml Optimal dilutions to be determined by the researcher.
• Grade: Affinity Purified
• Immunogen: Fusion protein corresponding to residues 1-100 of human beta-catenin with a C-terminal histidine tag. The immunizing sequence is identical in mouse and rat, has 99/100 identical amino acids in pig, 97/100 identical amino acids in frog and chicken, and 91/100 identical amino acids in zebrafish.
• Preparation and Storage: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

WB (Western Blot)

(WB: A431 cell lysate was probed with anti-Catenin, beta, active (ABC). Arrow indicates beta-Catenin (92kD))

Related Product Information for anti-CTNNB1 antibody

Catenins are proteins found in complexes with cadherin cell adhesion molecules of animal cells. The first two catenins that were identified became known as alpha-catenin and beta-catenin. alpha-catenin can bind to beta-catenin and can also bind actin. beta-catenin binds the cytoplasmic domain of some cadherins. When beta-catenin was sequenced it was found to be a member of the armadillo family of proteins. These proteins have multiple copies of the so-called armadillo repeat domain which is specialized for protein-protein binding. An increase in beta-catenin production has been noted in those people who have Basal Cell Carcinoma and leads to the increase in proliferation of related tumors. When beta-catenin is not associated with cadherins and alpha-catenin, it can interact with other proteins such as Catenin Beta Interacting Protein 1 (ICAT) and adenomatosis polyposis coli (APC). Recent evidence suggests that beta-catenin plays an important role in various aspects of liver biology including liver development (both embryonic and postnatal), liver regeneration following partial hepatectomy. HGF-induced hepatpomegaly, liver zonation, and pathogenesis of liver cancer.

Product Categories/Family for anti-CTNNB1 antibody

Antibodies; Abs to Catenin

NCBI and Uniprot Product Information

• NCBI GI #: 115497488
• NCBI GeneID: 539003
• NCBI Accession #: NP_001069609.1
• NCBI GenBank Nucleotide #: NM_001076141.1
• UniProt Accession #: Q0VCX4; A7E3R2
• NCBI Official Full Name: catenin beta-1
• NCBI Official Synonym Full Names: catenin (cadherin-associated protein), beta 1, 88kDa<
• NCBI Official Symbol: CTNNB1
• NCBI Protein Information: catenin beta-1; beta-catenin
• UniProt Protein Name: Catenin beta-1
• UniProt Gene Name: CTNNB1
• UniProt Entry Name: CTNB1_BOVIN