WB (Western Blot)
(beta-Catenin antibody (pAb) tested by Western blot. Detection of beta-Catenin antibody by Western blot analysis. Lane 1: A549 nuclear extract (30 ug). Lane 2: DU145 nuclear extract (20 ug). Both probed with beta-Catenin antibody at a dilution of 1:500.)
Rabbit anti-Human beta-Catenin Polyclonal Antibody | anti-beta-Catenin antibody
beta-Catenin antibody (pAb)
Gene Names
CTNNB1; CTNNB; MRD19; armadillo
Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Purity
Affinity Purified
Synonyms
beta-Catenin, Antibody; beta-Catenin antibody (pAb); CTNNB; MRD19; armadillo; anti-beta-Catenin antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-beta-Catenin antibody
WB (Western Blot), IP (Immunoprecipitation)
Immunogen
This antibody was raised against a peptide within the N-terminal region of human beta-Catenin.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
WB (Western Blot)
(beta-Catenin antibody (pAb) tested by Western blot. Detection of beta-Catenin antibody by Western blot analysis. Lane 1: A549 nuclear extract (30 ug). Lane 2: DU145 nuclear extract (20 ug). Both probed with beta-Catenin antibody at a dilution of 1:500.)
WB (Western Blot)
(beta-Catenin antibody (pAb) tested by Western blot. Detection of beta-Catenin antibody by Western blot analysis. Lane 1: A549 nuclear extract (30 ug). Lane 2: DU145 nuclear extract (20 ug). Both probed with beta-Catenin antibody at a dilution of 1:500.)
WB (Western Blot)
(beta-Catenin antibody (pAb) tested by Immunoprecipitation. 10 ul of beta-Catenin antibody was used to immunoprecipitate beta-Catenin from 500 ug of HeLa whole cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the beta-Catenin antibody at a dilution of 1:500.)
WB (Western Blot)
(beta-Catenin antibody (pAb) tested by Immunoprecipitation. 10 ul of beta-Catenin antibody was used to immunoprecipitate beta-Catenin from 500 ug of HeLa whole cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the beta-Catenin antibody at a dilution of 1:500.)
Related Product Information for anti-beta-Catenin antibody
Short Description: Our beta-Catenin antibody (pAb) was raised in a Rabbit host. It has been validated for use in Immunoprecipitation and Western blot; it has been shown to react with Human samples.
Background: beta-Catenin is a key downstream component of the canonical Wnt signaling pathway, plays an important role in the regulation of cell adhesion and embryonic development. In the absence of Wnt, it forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of beta-Catenin via BTRC and subsequent degradation by the proteasome. In the presence of Wnt ligand, beta-Catenin is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activation of Wnt responsive genes.
Background: beta-Catenin is a key downstream component of the canonical Wnt signaling pathway, plays an important role in the regulation of cell adhesion and embryonic development. In the absence of Wnt, it forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of beta-Catenin via BTRC and subsequent degradation by the proteasome. In the presence of Wnt ligand, beta-Catenin is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activation of Wnt responsive genes.
Product Categories/Family for anti-beta-Catenin antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
9,501 Da
NCBI Official Full Name
catenin beta-1
NCBI Official Synonym Full Names
catenin (cadherin-associated protein), beta 1, 88kDa
NCBI Official Symbol
CTNNB1
NCBI Official Synonym Symbols
CTNNB; MRD19; armadillo
NCBI Protein Information
catenin beta-1
UniProt Protein Name
Catenin beta-1
UniProt Gene Name
CTNNB1
UniProt Synonym Gene Names
CTNNB
UniProt Entry Name
CTNB1_HUMAN
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Product Notes
The beta-Catenin ctnnb1 (Catalog #AAA60033) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The beta-Catenin antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's beta-Catenin can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the beta-Catenin ctnnb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "beta-Catenin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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