FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing Hela cells stained with AAA279337 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1:200 dilution for 35min at 4 degree C.Control antibody (green line) was mouse IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
CAV1 recombinant antibody
CAV1 Recombinant Monoclonal Antibody
Gene Names
CAV1; CGL3; BSCL3; VIP21; MSTP085
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Functional Assay, Western Blot, ELISA
Purity
Affinity chromatography
Synonyms
CAV1, Recombinant Antibody; CAV1 Recombinant Monoclonal Antibody; CAV1; BSCL3 antibody; CAV antibody; CAV1 antibody; CAV1_HUMAN antibody; caveolae protein, 22 kD antibody; caveolin 1 alpha isoform antibody; caveolin 1 beta isoform antibody; Caveolin 1 caveolae protein 22kDa antibody; Caveolin-1 antibody; Caveolin1 antibody; cell growth-inhibiting protein 32 antibody; CGL3 antibody; LCCNS antibody; MSTP085 antibody; OTTHUMP00000025031 antibody; PPH3 antibody; VIP 21 antibody; VIP21 antibody; CAV1 recombinant antibody
Host
HEK293F Cell
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
mIgG2a
Clone Number
24F6
Purity/Purification
Affinity chromatography
Form/Format
Liquid; 0.03% Proclin 300, 50% glycerol, 0.01M PBS, pH 7.4.
Applicable Applications for CAV1 recombinant antibody
FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA
Antigen Species
Human
Immunogen
Recombinant Human CAV1 protein
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing Hela cells stained with AAA279337 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1:200 dilution for 35min at 4 degree C.Control antibody (green line) was mouse IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing Hela cells stained with AAA279337 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1:200 dilution for 35min at 4 degree C.Control antibody (green line) was mouse IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
WB (Western Blot)
(Western BlotPositive WB detected in:PC-3 whole cell lysate (20ug), A549 whole cell lysate (20ug), U251 whole cell lysate (20ug), Hela whole cell lysate (20ug), MCF-7 whole cell lysate (20ug), HEK293 whole cell lysate (20ug), Mouse Heart tissue lysate (20ug), Rat Heart tissue lysate (20ug)All lanes: CAV1 antibody at 1:500SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size:20 kDaObserved band size:20 kDaExposure time:120s)
WB (Western Blot)
(Western BlotPositive WB detected in:PC-3 whole cell lysate (20ug), A549 whole cell lysate (20ug), U251 whole cell lysate (20ug), Hela whole cell lysate (20ug), MCF-7 whole cell lysate (20ug), HEK293 whole cell lysate (20ug), Mouse Heart tissue lysate (20ug), Rat Heart tissue lysate (20ug)All lanes: CAV1 antibody at 1:500SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size:20 kDaObserved band size:20 kDaExposure time:120s)
Product Categories/Family for CAV1 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20,472 Da
NCBI Official Full Name
caveolin-1 isoform alpha
NCBI Official Synonym Full Names
caveolin 1, caveolae protein, 22kDa
NCBI Official Symbol
CAV1
NCBI Official Synonym Symbols
CGL3; BSCL3; VIP21; MSTP085
NCBI Protein Information
caveolin-1; cell growth-inhibiting protein 32
UniProt Protein Name
Caveolin-1
UniProt Gene Name
CAV1
UniProt Synonym Gene Names
CAV
UniProt Entry Name
CAV1_HUMAN
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Product Notes
The CAV1 cav1 (Catalog #AAA279337) is a Recombinant Antibody produced from HEK293F Cell and is intended for research purposes only. The product is available for immediate purchase. The CAV1 Recombinant Monoclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CAV1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the CAV1 cav1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CAV1, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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