ELISA
(The Binding Activity of CCR8 with Anti-CCR8 recombinant antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CCR8 at 5 ug/mL can bind Anti-CCR8 recombinant antibody, the EC50 is 11.20-15.63 ng/mL.)
CCR8 recombinant antibody
CCR8 Recombinant Monoclonal Antibody
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA
Purity
Affinity-chromatography
Synonyms
CCR8, Recombinant Antibody; CCR8 Recombinant Monoclonal Antibody; CCR8; CKRL1; CMKBR8; CMKBRL2; C-C chemokine receptor type 8; C-C CKR-8; CC-CKR-8; CCR-8; CC chemokine receptor CHEMR1; Chemokine receptor-like 1; CKR-L1; GPR-CY6; GPRCY6; TER1; CD antigen CDw198; CCR8 recombinant antibody
Reactivity
Human
Clonality
Monoclonal
Isotype
hIgG1
Clone Number
6B12
Purity/Purification
Affinity-chromatography
Form/Format
Liquid
Applicable Applications for CCR8 recombinant antibody
FCM/FACS (Flow Cytometry), ELISA
Immunogen
Recombinant Human CCR8 protein
Immunogen Species
Homo Sapiens (Human)
Conjugate
Non-conjugated
Research Area
Immunology; Microbiology
Buffer
Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Preparation and Storage
Store at -20°C or -80°C. Avoid repeated freeze.
ELISA
(The Binding Activity of CCR8 with Anti-CCR8 recombinant antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CCR8 at 5 ug/mL can bind Anti-CCR8 recombinant antibody, the EC50 is 11.20-15.63 ng/mL.)
ELISA
(The Binding Activity of CCR8 with Anti-CCR8 recombinant antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CCR8 at 5 ug/mL can bind Anti-CCR8 recombinant antibody, the EC50 is 11.20-15.63 ng/mL.)
FCM/FACS (Flow Cytometry)
(Untransfected CHO-K1 cells (green line) and transfected Human CCR8 CHO-K1 stable cells (red line) were stained with anti-CCR8 antibody (2ug/1*106cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.)
FCM/FACS (Flow Cytometry)
(Untransfected CHO-K1 cells (green line) and transfected Human CCR8 CHO-K1 stable cells (red line) were stained with anti-CCR8 antibody (2ug/1*106cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.)
Related Product Information for CCR8 recombinant antibody
The synthesis of the CCR8 recombinant monoclonal antibody is carried out with meticulous precision to ensure its exceptional quality and specificity. The process begins by isolating B cells from the spleen of an immunized animal, where the recombinant human CCR8 protein serves as the immunogen. RNA is extracted from the B cells and converted into cDNA through reverse transcription. Using specific primers designed for the antibody constant regions, the CCR8 antibody genes are amplified and inserted into an expression vector. The vector is then introduced into host cells through transfection, allowing for the production of the CCR8 recombinant monoclonal antibody. After a period of cell culture, the antibody is harvested from the cell culture supernatant and purified using affinity chromatography, resulting in a highly purified form suitable for a wide range of applications. To ensure its reliability and effectiveness, the antibody undergoes FC analysis to validate its specificity and functionality in detecting human CCR8 protein.
NCBI and Uniprot Product Information
UniProt Accession #
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Product Notes
The CCR8 (Catalog #AAA278952) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The CCR8 Recombinant Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CCR8 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA. Researchers should empirically determine the suitability of the CCR8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CCR8, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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