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FCM/FACS (Flow Cytometry)
(Flow cytometry: 1X10^6 293T (Transfection) cells were surface-stained with Rabbit IgG isotype control (AC042,2 ug/mL,left) or CD10 Rabbit mAb (AAA283065,2 ug/mL,right), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining.)
Rabbit anti-Mouse CD10 Monoclonal Antibody | anti-CD10 antibody
CD10 Rabbit mAb
Reactivity
Mouse
Applications
ELISA, Flow Cytometry, Functional Assay, Western Blot
Purity
Affinity purification
Synonyms
CD10, Antibody; CD10 Rabbit mAb; Mme; NEP; SFE; CD10; CALLA; 6030454K05Rik; anti-CD10 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Sequence
YDDGICKSSDCIKSAARLIQNMDASVEPCTDFFKYACGGWLKRNVIPETSSRYSNFDILRDELEVILKDVLQEPKTEDIVAVQKAKTLYRSCINESAIDSRGGQPLLKLLPDIYGWPVASDNWDQTYGTSWTAEKSIAQLNSKYGKKVLINFFVGTDDKNSTQHIIHFDQPRLGLPSRDYYECTGIYKEACTAYVDFMISVARLIRQEQSLPIDENQLSLEMNKVMELEKEIANATTKPEDRNDPMLLYNKMTLAKLQNNFSLEVNGKSFSWSNFTNEIMSTVNINIQNEEEVVVYAPEYLTKLKPILTKYSPRDLQNLMSWRFIMDLVSSLSRNYKESRNAFRKALYGTTSETATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLIAQIREVFIQTLDDLTWMDAETKKKAEEKALAIKERIGYPDDIISNENKLNNEYLELNYREDEYFENIIQNLKFSQSKQLKKLREKVDKDEWISGAAVVNAFYSSGRNQIVFPAGILQPPFFSAQQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSANNFKDQSQCMVYQYGNFSWDLAGGQHLNGINTLGENIADNGGIGQAYRAYQNYVKKNGEEKLLPGLDLNHKQLFFLNFAQVWCGTYRPEYAVNSIKTDVHSPGNFRIIGTLQNSAEFADAFHCRKNSYMNPERKCRVW
Applicable Applications for anti-CD10 antibody
ELISA, FCM/FACS (Flow Cytometry), WB (Western Blot)
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 52-750 of mouse CD10 (NP_032630.2).
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
FCM/FACS (Flow Cytometry)
(Flow cytometry: 1X10^6 293T (Transfection) cells were surface-stained with Rabbit IgG isotype control (AC042,2 ug/mL,left) or CD10 Rabbit mAb (AAA283065,2 ug/mL,right), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining.)
FCM/FACS (Flow Cytometry)
(Flow cytometry: 1X10^6 293T (Transfection) cells were surface-stained with Rabbit IgG isotype control (AC042,2 ug/mL,left) or CD10 Rabbit mAb (AAA283065,2 ug/mL,right), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining.)
FCM/FACS (Flow Cytometry)
(Flow cytometry: 1X10^6 293T cells (negative control,left) and 293T cells (right) were surface-stained with CD10 Rabbit mAb (AAA283065,2 ug/mL,orange line) or Rabbit IgG isotype control (AC042,2 ug/mL,blue line). followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).)
FCM/FACS (Flow Cytometry)
(Flow cytometry: 1X10^6 293T cells (negative control,left) and 293T cells (right) were surface-stained with CD10 Rabbit mAb (AAA283065,2 ug/mL,orange line) or Rabbit IgG isotype control (AC042,2 ug/mL,blue line). followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).)
WB (Western Blot)
(Western blot analysis of lysates from Mouse kidney using CD10 Rabbit mAb (AAA283065) at 1:1000 dilution incubated overnight at 4°C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020)Exposure time: 30 s.)
WB (Western Blot)
(Western blot analysis of lysates from Mouse kidney using CD10 Rabbit mAb (AAA283065) at 1:1000 dilution incubated overnight at 4°C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020)Exposure time: 30 s.)
WB (Western Blot)
(Western blot analysis of lysates from wild type (WT) and 293F cells transfected with CD10 using CD10 Rabbit mAb (AAA283065) at 1:3000 dilution incubated overnight at 4°C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 30 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020)Exposure time: 60 s.)
WB (Western Blot)
(Western blot analysis of lysates from wild type (WT) and 293F cells transfected with CD10 using CD10 Rabbit mAb (AAA283065) at 1:3000 dilution incubated overnight at 4°C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 30 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020)Exposure time: 60 s.)
Related Product Information for anti-CD10 antibody
Enables peptidase activity. Involved in amyloid-beta clearance; positive regulation of long-term synaptic potentiation; and sensory perception of pain. Acts upstream of or within amyloid-beta metabolic process. Located in several cellular components, including dendrite; neuron projection terminus; and synaptic vesicle. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; liver and biliary system; and mammary gland. Used to study Alzheimer's disease. Human ortholog(s) of this gene implicated in Alzheimer's disease; Charcot-Marie-Tooth disease axonal type 2T; cerebellar ataxia type 43; cerebral amyloid angiopathy; and membranous glomerulonephritis. Orthologous to human MME (membrane metalloendopeptidase).
Product Categories/Family for anti-CD10 antibody
NCBI and Uniprot Product Information
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Product Notes
The CD10 mme (Catalog #AAA283065) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD10 Rabbit mAb reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD10 can be used in a range of immunoassay formats including, but not limited to, ELISA, FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the CD10 mme for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: YDDGICKSSD CIKSAARLIQ NMDASVEPCT DFFKYACGGW LKRNVIPETS SRYSNFDILR DELEVILKDV LQEPKTEDIV AVQKAKTLYR SCINESAIDS RGGQPLLKLL PDIYGWPVAS DNWDQTYGTS WTAEKSIAQL NSKYGKKVLI NFFVGTDDKN STQHIIHFDQ PRLGLPSRDY YECTGIYKEA CTAYVDFMIS VARLIRQEQS LPIDENQLSL EMNKVMELEK EIANATTKPE DRNDPMLLYN KMTLAKLQNN FSLEVNGKSF SWSNFTNEIM STVNINIQNE EEVVVYAPEY LTKLKPILTK YSPRDLQNLM SWRFIMDLVS SLSRNYKESR NAFRKALYGT TSETATWRRC ANYVNGNMEN AVGRLYVEAA FAGESKHVVE DLIAQIREVF IQTLDDLTWM DAETKKKAEE KALAIKERIG YPDDIISNEN KLNNEYLELN YREDEYFENI IQNLKFSQSK QLKKLREKVD KDEWISGAAV VNAFYSSGRN QIVFPAGILQ PPFFSAQQSN SLNYGGIGMV IGHEITHGFD DNGRNFNKDG DLVDWWTQQS ANNFKDQSQC MVYQYGNFSW DLAGGQHLNG INTLGENIAD NGGIGQAYRA YQNYVKKNGE EKLLPGLDLN HKQLFFLNFA QVWCGTYRPE YAVNSIKTDV HSPGNFRIIG TLQNSAEFAD AFHCRKNSYM NPERKCRVW. It is sometimes possible for the material contained within the vial of "CD10, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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