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WB (Western Blot)
(Detection of human CD103/ITGAE by western blot. Samples: Whole cell lysate (50 ug) from C5/MJ, HeLa, CCRF-CEM, HEK293T, and MJ cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD103/ITGAE recombinant monoclonal antibody (AAA213625 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
CD103/ITGAE recombinant antibody
Rabbit anti-CD103/ITGAE Recombinant Monoclonal Antibody [BLR171J]
Gene Names
ITGAE; CD103; HUMINAE
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
CD103/ITGAE, Recombinant Antibody; Rabbit anti-CD103/ITGAE Recombinant Monoclonal Antibody [BLR171J]; CD103; HUMINAE; mucosal lymphocyte 1 antigen; integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1); integrin alpha-IEL; integrin alpha-E; HML-1 antigen; alpha polypeptide; CD antigen CD103; antigen CD103, human mucosal lymphocyte antigen 1; human mucosal lymphocyte antigen 1, alpha polypeptide; Integrin alpha-E; integrin subunit alpha E; CD103/ITGAE recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR171J
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Concentration
1000ug/ml (varies by lot)
Applicable Applications for CD103/ITGAE recombinant antibody
ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 425 and 475
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human CD103/ITGAE by western blot. Samples: Whole cell lysate (50 ug) from C5/MJ, HeLa, CCRF-CEM, HEK293T, and MJ cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD103/ITGAE recombinant monoclonal antibody (AAA213625 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot)
(Detection of human CD103/ITGAE by western blot. Samples: Whole cell lysate (50 ug) from C5/MJ, HeLa, CCRF-CEM, HEK293T, and MJ cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD103/ITGAE recombinant monoclonal antibody (AAA213625 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
IP (Immunoprecipitation)
(Detection of human CD103/ITGAE by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MJ cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD103/ITGAE recombinant monoclonal antibody (AAA213625 lot 1) used for IP at 6 ul/mg lysate. CD103/ITGAE was also immunoprecipitated by a second antibody against a different epitope of CD103/ITGAE (Antibody 2). For blotting immunoprecipitated CD103/ITGAE, AAA213625 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human CD103/ITGAE by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MJ cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD103/ITGAE recombinant monoclonal antibody (AAA213625 lot 1) used for IP at 6 ul/mg lysate. CD103/ITGAE was also immunoprecipitated by a second antibody against a different epitope of CD103/ITGAE (Antibody 2). For blotting immunoprecipitated CD103/ITGAE, AAA213625 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemisry)
(Detection of human CD103/ITGAE in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-CD103/ITGAE recombinant monoclonal antibody (AAA213625 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemisry)
(Detection of human CD103/ITGAE in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-CD103/ITGAE recombinant monoclonal antibody (AAA213625 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohiostchemistry)
(Detection of human CD103/ITGAE in FFPE tonsil by IHC. Antibody: Rabbit anti-CD103/ITGAE recombinant monoclonal antibody (AAA213625 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohiostchemistry)
(Detection of human CD103/ITGAE in FFPE tonsil by IHC. Antibody: Rabbit anti-CD103/ITGAE recombinant monoclonal antibody (AAA213625 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human CD103/ITGAE in FFPE C5/MJ cells by ICC. Antibody: Rabbit anti-CD103/ITGAE recombinant monoclonal antibody (AAA213625 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human CD103/ITGAE in FFPE C5/MJ cells by ICC. Antibody: Rabbit anti-CD103/ITGAE recombinant monoclonal antibody (AAA213625 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
Related Product Information for CD103/ITGAE recombinant antibody
Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. CD103/ITGAE is an I-domain-containing alpha integrin that undergoes post-translational cleavage in the extracellular domain, yielding disulfide-linked heavy and light chains. In combination with the beta 7 integrin, CD103/ITGAE forms the E-cadherin binding integrin known as the human mucosal lymphocyte-1 antigen, and is preferentially expressed in human intestinal intraepithelial T-lymphocytes (IEL). In addition to a role in adhesion, CD103/ITGAE may serve as an accessory molecule for IEL activation. [taken from NCBI Entrez Gene (Gene ID: 3682)].
Product Categories/Family for CD103/ITGAE recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
130,159 Da
NCBI Official Full Name
integrin alpha-E
NCBI Official Synonym Full Names
integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide)
NCBI Official Symbol
ITGAE
NCBI Official Synonym Symbols
CD103; HUMINAE
NCBI Protein Information
integrin alpha-E; HML-1 antigen; antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide; integrin alpha-IEL; mucosal lymphocyte 1 antigen
UniProt Protein Name
Integrin alpha-E
UniProt Gene Name
ITGAE
UniProt Entry Name
ITAE_HUMAN
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Product Notes
The CD103/ITGAE itgae (Catalog #AAA213625) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-CD103/ITGAE Recombinant Monoclonal Antibody [BLR171J] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD103/ITGAE can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the CD103/ITGAE itgae for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD103/ITGAE, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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