Application Data
(Figure A. A647 conjugated mouse anti porcine CD45 (please inquire) and A488 conjugated mouse IgG1 isotype control (please inquire).Figure B. A647 conjugated mouse anti porcine CD45 (please inquire) and A488 conjugated mouse anti porcine CD117 (please inquire). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.)
Mouse CD117 Monoclonal Antibody | anti-CD117 antibody
MOUSE ANTI PIG CD117
Gene Names
KIT; MGF
Applications
Western Blot, Immunoprecipitation, Flow Cytometry, Functional Assay, Immunohistochemistry
Synonyms
CD117, Antibody; MOUSE ANTI PIG CD117; anti-CD117 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2B8/BM
Form/Format
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (varies by lot)
Sequence Length
972
Applicable Applications for anti-CD117 antibody
WB (Western Blot), IP (Immunoprecipitation), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry)
Immunogen
Porcine bone marrow cells (BMC).
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Target Species
Pig
Carrier Free
Yes
Fusion Partners
Spleen cells from immunized Balb/c mouse were fused with cells of the Sp2/0 myeloma cell line.
Preparation and Storage
It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Data
(Figure A. A647 conjugated mouse anti porcine CD45 (please inquire) and A488 conjugated mouse IgG1 isotype control (please inquire).Figure B. A647 conjugated mouse anti porcine CD45 (please inquire) and A488 conjugated mouse anti porcine CD117 (please inquire). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.)
Application Data
(Figure A. A647 conjugated mouse anti porcine CD45 (please inquire) and A488 conjugated mouse IgG1 isotype control (please inquire).Figure B. A647 conjugated mouse anti porcine CD45 (please inquire) and A488 conjugated mouse anti porcine CD117 (please inquire). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.)
Application Data
(Porcine Lymphocytes stained with Mouse anti Porcine CD117: Alexa FluorR 488)
Application Data
(Porcine Lymphocytes stained with Mouse anti Porcine CD117: Alexa FluorR 488)
Related Product Information for anti-CD117 antibody
Mouse anti Pig CD117, clone 2B8/BM is specific for porcine CD117, also known as c-kit, a 155kDa type I transmembrane protein with protein tyrosine kinase activity, which plays an important role in early hematopoiesis (Perez et al. 2007). Hematopoietic stem cells (HSC) of bone marrow have multilineage differentiation potential and an extensive capacity for self-renewal. The majority of adult bone marrow hematopoietic progenitor cells are CD117+ and have been used successfully in xenograft transplantation models for long term survival of grafts, without symptoms of graft-versus-host disease (GVHD). Mouse anti pig CD117, clone 2B8/BM recognizes CD117 on a small subset of porcine bone marrow progenitor cells and therefore provides an alternative tool from the previously used c-kit ligand stem cell factor, for the isolation and enrichment of porcine stem cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
mast/stem cell growth factor receptor Kit
NCBI Official Symbol
KIT
NCBI Official Synonym Symbols
MGF
NCBI Protein Information
mast/stem cell growth factor receptor Kit; SCFR; c-kit; proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit
UniProt Protein Name
Mast/stem cell growth factor receptor Kit
UniProt Gene Name
KIT
UniProt Synonym Gene Names
SCFR
UniProt Entry Name
KIT_PIG
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Product Notes
The CD117 kit (Catalog #AAA50134) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD117 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IP (Immunoprecipitation), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the CD117 kit for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD117, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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