IHC (Immunohiostchemistry)
(Immunohistochemistry: Human lymphomacarcinoma (FFPE) stained with Rabbit anti-CD117/kit (Cat# AAA71440) at 1:200 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
Rabbit CD117 (kit) Polyclonal Antibody | anti-CD117 (kit) antibody
Rabbit anti CD117/c-Kit (CT) Polyclonal antibody
Reactivity
Human, Mouse, Rat, Canine
Applications
Immunoprecipitation, ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
The Rabbit IgG is purified by Epitope Affinity Purification.
Synonyms
CD117 (kit), Antibody; Rabbit anti CD117/c-Kit (CT) Polyclonal antibody; Rabbit anti--c-Kit (CT); Kit Protein; c-Kit; CD117 antigen; SCFR; PBT; anti-CD117 (kit) antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Canine
Clonality
Polyclonal
Specificity
This antibody recognizes CD117/c-Kit protein. It cross- reacts to human, mouse and rat. The other species were not tested.
Purity/Purification
The Rabbit IgG is purified by Epitope Affinity Purification.
Form/Format
This affinity purified antibody is supplied in sterile Phosphate-buffered saline (pH7.2) containing antibody stabilizer.
Applicable Applications for anti-CD117 (kit) antibody
IP (Immunoprecipitation), ELISA, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot)
Antigen Preparation
A synthetic peptide derived from N-terminus of Kit protein. This sequence is identical in human, rat, mouse and dog origins.
Positive Control
Kidney Tissue
Cellular Location
Cell Membrane
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C.
The antibodies can be stored at 2 degree C to 8 degree C for three months without detectable loss of activity. Avoid repeated freezing-thawing cycles.
The antibodies can be stored at 2 degree C to 8 degree C for three months without detectable loss of activity. Avoid repeated freezing-thawing cycles.
IHC (Immunohiostchemistry)
(Immunohistochemistry: Human lymphomacarcinoma (FFPE) stained with Rabbit anti-CD117/kit (Cat# AAA71440) at 1:200 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
IHC (Immunohiostchemistry)
(Immunohistochemistry: Human lymphomacarcinoma (FFPE) stained with Rabbit anti-CD117/kit (Cat# AAA71440) at 1:200 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
WB (Western Blot)
(Western Blot: The whole cell lysate derived from Jurkat cell was separated in 10% SDS-PAGE, transferred onto NC membrane, and immunoblotted by Rabbit anti–CD117/c-Kit (CT) antibody (Cat#AAA71440) at 1:500 . An immunoreactive band around ~145 kDa was observed.)
WB (Western Blot)
(Western Blot: The whole cell lysate derived from Jurkat cell was separated in 10% SDS-PAGE, transferred onto NC membrane, and immunoblotted by Rabbit anti–CD117/c-Kit (CT) antibody (Cat#AAA71440) at 1:500 . An immunoreactive band around ~145 kDa was observed.)
Related Product Information for anti-CD117 (kit) antibody
Background: The c-Kit proto-oncogene encodes a 145 kDa transmembrane receptor (CD117) with tyrosine kinase activity that is structurally related to platelet-derived growth factor. C-Kit/CD117 antigen is expressed on almost all hematopoietic stem and progenitor cells. c-Kit regulates a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion. Ligand binding to the extracellular domain leads to autophosphorylation on several tyrosine residues within the cytoplasmic domain and activation. Mutations in c-Kit have been found to be important for tumor growth and progression in a variety of cancers including mast cell diseases, gastrointestinal stromal tumor, acute myeloid leukemia, Ewing sarcoma and lung cancer. C-kit plays an important role in hematopoiesis, melanogenesis, and gametogenesis.
References
Li J et al. Expression of c-Kit receptor tyrosine kinase and effect on beta-cell development in the human fetal pancreas. Am J Physiol Endocrinol Metab 293:E475-83 (2007).
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Product Notes
The CD117 (kit) (Catalog #AAA71440) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti CD117/c-Kit (CT) Polyclonal antibody reacts with Human, Mouse, Rat, Canine and may cross-react with other species as described in the data sheet. AAA Biotech's CD117 (kit) can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ELISA, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CD117 (kit) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD117 (kit), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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